HUMAN COLONIC STEM-CELL MUTATION FREQUENCY WITH AND WITHOUT IRRADIATION
HUMAN COLONIC STEM-CELL MUTATION FREQUENCY WITH AND WITHOUT IRRADIATION
复制标题
DOI：
10.1002/path.1711740306
复制
发表时间：
1994-11-01
影响因子：
7.3
通讯作者：
WILLIAMS, ED
中科院分区：
文献类型：
--
作者：
CAMPBELL, F;FULLER, CE;WILLIAMS, ED
轻度周期性酸-希夫（mPAS）染色区分o -乙酰化和非o -乙酰化唾液糖蛋白。在人类结肠粘膜中，个体具有三种表型之一：均匀mpas阳性（非o -乙酰化），均匀mpas阴性（o -乙酰化），阴性伴少见的分散阳性隐窝。这是由于单个常染色体基因（燕麦）的多态性。在儿童结肠中未发现不一致的隐窝，这表明它们是由杂合个体的体细胞突变引起的。我们现在根据对辐射引起的变化的研究提出证据来证实这一点。直肠癌术前4周接受放射治疗的患者与单独接受手术的对照组的大肠粘膜mPAS染色比较显示相似的表型分布，但信息照射的患者显示不一致隐窝的频率增加(照射vs，未照射14.5 +/- 8.2 x 10（-4) vs 6.1 +/- 4.2 x 10(-4)）。当这些被分类为完全或部分参与异常表型时，部分参与隐窝的增加最为明显(7.5 +/- 4.5 x 10(-4) vs. 0.3 +/- 0.5 x 10（-4）
Mild periodic acid-Schiff (mPAS) staining distinguishes O-acetylated from non-O-acetylated sialoglycoproteins. In human colonic mucosa, individuals possess one of three phenotypes: uniformly mPAS-positive (non-O-acetylated), uniformly mPAS-negative (O-acetylated), and negative with infrequent scattered positive crypts. This is due to a polymorphism in a single autosomal gene (oat). Discordant crypts have not been found in children's colons, suggesting that they result from somatic mutation in heterozygous individuals. We now present evidence to confirm this based on a study of radiation-induced changes. Comparison of mPAS staining of large intestinal mucosa from patients given radiation 4 weeks before surgery for carcinoma of the rectum with matched controls receiving surgery alone showed a similar phenotype distribution, but informative irradiated patients showed an increased frequency of discordant crypts (irradiated vs, non-irradiated 14.5 +/- 8.2 x 10(-4) vs. 6.1 +/- 4.2 x 10(-4)). When these were classified as wholly or partially involved by the aberrant phenotype, the increase was most marked in partially involved crypts (7.5 +/- 4.5 x 10(-4) vs. 0.3 +/- 0.5 x 10(-4), Mann-Whitney U, P