Ectoderm cell--ECM interaction is essential for sea urchin embryo skeletogenesis.
Ectoderm cell--ECM interaction is essential for sea urchin embryo skeletogenesis.
复制标题
外胚层细胞-ECM 相互作用对于海胆胚胎骨骼形成至关重要。
DOI：
10.1006/dbio.1998.8866
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发表时间：
1998
影响因子：
2.7
通讯作者：
V. Matranga
中科院分区：
文献类型：
--
作者：
F. Zito;V. Tesoro;D. McClay;E. Nakano;V. Matranga
Paracentrotus lividus 海胆连接蛋白 (Pl-nectin) 是海胆胚胎外胚层顶面的细胞外基质 (ECM) 蛋白，已被证明是胚胎细胞的粘附基质。产生了针对 Pl-nectin 的单克隆抗体 (McAb)，该抗体在体外功能测定中抑制囊胚细胞与 Pl-nectin 包被的基质的粘附。为了检查 Pl-nectin 可能的体内功能，将 Pl-nectin McAb 的 Fab 片段 (Fab) 添加到早期囊胚中。原代间充质细胞的侵入不受 Fab 的影响。当对照胚胎达到臀肌阶段时，处理过的胚胎表现出骨骼伸长和模式的严重抑制。当 Fab 直接注射到囊胚腔中时，即使浓度高于外部注射的浓度，骨骼形成也是正常的。因此，抗体对针发生的影响是间接的。这种治疗是部分可逆的，因为胚胎最终似乎恢复并拉长了针状体，尽管其模式不正确。色素细胞的迁移也受到 Fab 的影响，因为它们没有分散在整个外胚层中，而是仍然聚集在异位区域。相反，内胚层结构的发育不受影响。我们的结果表明，在海胆胚胎中，外胚层细胞与外部 ECM 成分的适当接触对于内部中胚层结构的正确形态发生至关重要。
Paracentrotus lividus sea urchin nectin (Pl-nectin) is an extracellular matrix (ECM) protein of the sea urchin embryo on the apical surface of the ectoderm and has been shown to be an adhesive substrate for embryonic cells. A monoclonal antibody (McAb) to Pl-nectin was generated that inhibits the adhesion of blastula cells to Pl-nectin-coated substrates in an in vitro functional assay. To examine for possible in vivo functions of Pl-nectin, Fab fragments (Fabs) of Pl-nectin McAb were added to early blastulae. Ingression of primary mesenchyme cells was not affected by Fabs. As control embryos reached the pluteus stage, treated embryos showed a severe inhibition of skeletal elongation and patterning. When the Fabs were injected directly into the blastocoel, even at higher concentration than was applied externally, skeletogenesis was normal. Therefore, the effect of the antibody on spiculogenesis was indirect. The treatment was partially reversible as embryos eventually seemed to recover and elongate spicules, although with an incorrect patterning. Migration of pigment cells was also affected by the Fabs, since they did not disperse throughout the ectoderm but remained clustered in ectopic areas. In contrast, the development of endoderm structures was not affected. Our results indicate that in the sea urchin embryo the appropriate contact of ectodermal cells with outer ECM components is essential for the correct morphogenesis of inner mesodermal structures.