Inhibitory effect of Pseudomonas putida nitrogen-related phosphotransferase system on conjugative transfer of IncP-9 plasmid from Escherichia coli
Inhibitory effect of Pseudomonas putida nitrogen-related phosphotransferase system on conjugative transfer of IncP-9 plasmid from Escherichia coli
复制标题
恶臭假单胞菌氮相关磷酸转移酶系统对大肠杆菌IncP-9质粒接合转移的抑制作用
DOI：
10.1111/1574-6968.12188
复制
发表时间：
2013
影响因子：
2.1
通讯作者：
Tsuda M
中科院分区：
文献类型：
--
作者：
Inoue K;Miyazaki R;Ohtsubo Y;Nagata Y;Tsuda M
接合质粒转移系统已得到充分研究，但对控制水平传播的受体因素知之甚少。自传播 IncP-9 萘分解代谢质粒 NAH7 携带 traF 基因，其产物被认为是 NAH7 的宿主范围修饰剂，因为其 traF 缺失突变体 (NAH7dF) 可从恶臭假单胞菌 (Pseudomonas putidatoP) 传播。恶臭菌和大肠杆菌大肠杆菌E.大肠杆菌，但不是来自大肠杆菌。大肠杆菌P.恶臭。在这项研究中，P. 的转座子诱变。进行 putidaKT2440 来分离可以从大肠杆菌中接收 NAH7dF 的突变体。大肠杆菌。突变体在ptsPorptsO中插入了转座子，编码氮相关磷酸转移酶系统(PTSNtr)的三个组成部分中的两个。缺乏ptsN的KT2440衍生物编码PTSNtr的剩余成分，也能够接收NAH7dF。这些结果表明 PTSNtrinP。恶臭菌参与抑制来自大肠杆菌的 NAH7dF 接合转移。大肠杆菌。我们使用定点突变体进行的进一步实验表明，磷酸化形式的 PtsO 间接参与了接合转移的抑制。 NAH7 和另一个 IncP-9 质粒 pWW0（来自大肠杆菌）的接合转移。 KT2440 中的 PtsO 功能部分抑制了大肠杆菌。
Conjugative plasmid transfer systems have been well studied, but very little is known about the recipient factors that control horizontal transmission. A self-transmissible IncP-9 naphthalene catabolic plasmid, NAH7, carries thetraFgene, whose product is considered to be a host-range modifier of NAH7, because itstraFdeletion mutant (NAH7dF) is transmissible fromPseudomonas putidatoP. putidaandEscherichia coliand fromE. colitoE. coli, but not fromE. colitoP. putida. In this study, transposon mutagenesis ofP. putidaKT2440 was performed to isolate the mutants that could receive NAH7dF fromE. coli. The mutants had the transposon insertions inptsPorptsO, encoding two of three components of the nitrogen-related phosphotransferase system (PTSNtr). The KT2440 derivative lackingptsN, encoding the remaining component of PTSNtr, was also able to receive NAH7dF. These results indicated that the PTSNtrinP. putidais involved in inhibition of conjugative transfer of NAH7dF fromE. coli. Our further experiments using site-directed mutants suggested the indirect involvement of the phosphorylated form of PtsO in the inhibition of the conjugative transfer. Conjugative transfer of NAH7 and another IncP-9 plasmid, pWW0, fromE. coliwas partially inhibited by the PtsO function in KT2440.