Determination of 20 underivatized proteinic amino acids by ion-pairing chromatography and pneumatically assisted electrospray mass spectrometry
Determination of 20 underivatized proteinic amino acids by ion-pairing chromatography and pneumatically assisted electrospray mass spectrometry
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DOI：
10.1016/s0021-9673(99)00685-8
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发表时间：
1999-09-03
影响因子：
4.1
通讯作者：
Dreux, M
中科院分区：
文献类型：
--
作者：
Chaimbault, P;Petritis, K;Dreux, M
本文报道了20种未衍生化蛋白质氨基酸的LC-MS定性分析方法。通过对几种具有相似特征的氨基酸对的研究，证明了在质谱测定前进行色谱分离的必要性。提出了两种适用于氨基酸分析的LC-MS系统。由于这两种检测模式具有相同的色谱要求，因此使用蒸发光散射检测器对这些系统进行了初步优化。采用Purospher RP-18 e或Supelcosil ABZ（+）Plus色谱柱，以十三氟庚酸或十五氟辛酸为挥发性离子对试剂，乙腈-水移动的为流动相，实现了氨基酸的分离。为了去除保留最多的氨基酸，使用基于同时增加乙腈浓度和降低离子配对试剂浓度的洗脱梯度。本工作的检测限（没有专门的优化）从0.5到1毫克升（-1）。(C)1999年由Elsevier Science B. V.出版，版权所有。
A qualitative determination of 20 underivatized proteinic amino acids by LC-MS is reported. The need for chromatographic separation before mass spectrometry determination is demonstrated based on the study of several amino acid pairs which have some similar characteristics. Two suitable LC-MS systems are proposed for amino acid analysis. A preliminary optimization of these systems has been investigated using evaporative light scattering detection as these two detection modes have the same chromatographic requirements. The amino acid separation was achieved on a Purospher RP-18e or a Supelcosil ABZ(+)Plus column with tridecafluoroheptanoic acid or pentadecafluorooctanoic acid as volatile ion-pairing reagent in an acetonitrile-water mobile phase. In order to elute the most retained amino acids, an elution gradient based on simultaneously increasing the concentration of acetonitrile and decreasing the concentration of the ion-pairing reagent was used. The detection limits of the present work (without specialized optimization) varied from 0.5 to 1 mg l(-1). (C) 1999 Published by Elsevier Science B.V. All rights reserved.