A Highly Selective Electrochemical DNA-Based Sensor That Employs Steric Hindrance Effects to Detect Proteins Directly in Whole Blood
A Highly Selective Electrochemical DNA-Based Sensor That Employs Steric Hindrance Effects to Detect Proteins Directly in Whole Blood
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DOI：
10.1021/jacs.5b04942
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发表时间：
2015-12-23
影响因子：
15
通讯作者：
Vallee-Belisle, Alexis
中科院分区：
文献类型：
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作者：
Mahshid, Sahar Sadat;Camire, Sebastien;Vallee-Belisle, Alexis
在这里，我们描述了一个高度选择性的基于DNA的电化学传感器，利用空间位阻效应的信号存在的大分子在一个单步程序。我们首先表明，一个大的大分子，如蛋白质，当绑定到一个信号DNA链产生空间位阻效应，这限制了这种DNA杂交的能力，表面附着的互补链。我们证明了这种信号DNA的杂交效率与附着在其上的分子的大小呈负相关，遵循半对数关系。使用这种电化学形式的空间位阻杂交测定（eSHHA），我们证明了在低纳摩尔范围内对各种大分子的多重、定量、一步检测，
Here we describe a highly selective DNA-based electrochemical sensor that utilizes steric hindrance effects to signal the presence of large macromolecules in a single-step procedure. We first show that a large macromolecule, such as a protein, when bound to a signaling DNA strand generates steric hindrance effects, which limits the ability of this DNA to hybridize to a surface-attached complementary strand. We demonstrate that the efficiency of hybridization of this signaling DNA is inversely correlated with the size of the molecule attached to it, following a semilogarithmic relationship. Using this steric hindrance hybridization assay in an electrochemical format (eSHHA), we demonstrate the multiplexed, quantitative, one-step detection of various macromolecules in the low nanomolar range, in