DIAGNOSTIC ALLOZYMES FOR ROUTINE IDENTIFICATION OF ADULTS OF ANOPHELES-GAMBIAE COMPLEX (DIPTERA, CULICIDAE)
DIAGNOSTIC ALLOZYMES FOR ROUTINE IDENTIFICATION OF ADULTS OF ANOPHELES-GAMBIAE COMPLEX (DIPTERA, CULICIDAE)
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DOI：
10.1017/s0007485300006465
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发表时间：
1976-01-01
影响因子：
1.9
通讯作者：
HUNT, RH
中科院分区：
文献类型：
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作者：
MAHON, RJ;GREEN, CA;HUNT, RH
对南部非洲4种冈比亚蠓复合体的自然种群进行了取样和卵巢多染色体鉴定（A种19种，B种190种，C种140种，梅勒斯蠓54种）。经电泳鉴定，3个位点编码非特异性酯酶，1个位点编码四氮唑氧化酶。在四氮氧化酶位点没有种内变异；物种A、B和C有共同的同工酶，而A. merus是独一无二的。每个物种的3个酯酶位点的16个同酶的相对基因频率在最坏的采样条件下，即样品由相等的物种混合物组成，正确鉴定的最小概率为0.9533。在实践中，只有在物种A和物种c之间的区分中才需要使用这种概率。这种识别系统优于染色体方法，因为所有的成虫，无论是雄性还是雌性，无论处于性生殖周期的哪个阶段，都是可识别的。
Natural populations of 4 spp. of the A. gambiae complex occurring in southern Africa were sampled and individuals identified using ovarian polytene chromosomes (19 species A, 190 species B, 140 species C and 54 A. merus Don.). The genotypes of these same insects were scored for 3 loci coding for non-specific esterases, and 1 locus coding for tetrazolium oxidase, after electrophoresis. There is no intraspecific variation at the tetrazolium oxidase locus; species A, B and C share a common allozyme and A. merus is unique. The relative gene frequencies for the 16 allozymes of the 3 esterase loci for each species give a minimum probability of correct identification of 0.9533 under the worst conditions of sampling, i.e., where samples consist of equal mixtures of species. In practice, resort to such probabilities is necessary only in the discrimination between species A and C. This system of identification is superior to the chromosome method because all adults, whether male or female, and at whatever stage of the gonotropic cycle, are identifiable.