A Bioorthogonal Raman Reporter Strategy for SERS Detection of Glycans on Live Cells
A Bioorthogonal Raman Reporter Strategy for SERS Detection of Glycans on Live Cells
复制标题
用于活细胞上聚糖检测的生物正交拉曼报告策略
DOI：
10.1002/anie.201301387
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发表时间：
2013-07-08
影响因子：
16.6
通讯作者：
Chen, Xing
中科院分区：
文献类型：
--
作者：
Lin, Liang;Tian, Xiangdong;Chen, Xing
一般方法和材料。所有化学试剂均从Sigma-Aldrich和J&K获得，无需进一步纯化。分析薄层色谱（TLC）在玻璃背板Analtech Uniplate硅胶板上进行，并用铈钼酸铵（CAM）或50% H2SO4在MeOH中染色来观察化合物。使用Merck 60 Å 200-300目硅胶进行快速色谱。1H NMR和13C NMR光谱在Bruker AVANCE III 500 MHz或300 MHz光谱仪上获得，在CD3OH或D2O中使用四甲基硅烷（TMS）作为内标。利用北京大学分析仪器中心的Bruker APEX IV型傅立叶变换离子回旋共振质谱仪获得了电喷雾电离质谱（ESI）和高分辨率电喷雾电离质谱（HR-ESI-MS）。
General methods and materials. All chemical reagents were obtained from Sigma-Aldrich and J&K and used without further purification. Analytical thin layer chromatography (TLC) was performed on glass-backed Analtech Uniplate silica gel plates, and compounds were visualized by staining with Ceric ammonium molybdate (CAM) or 50% H2SO4 in MeOH. Flash chromatography was performed using Merck 60 Å 200-300 mesh silica gel. 1H NMR and 13C NMR spectra were obtained on a Bruker AVANCE III 500 MHz or 300 MHz spectrometer in CD3OH or D2O using tetramethylsilane (TMS) as an internal standard. Electrospray ionization (ESI) mass spectra and high resolution electrospray ionization mass spectra (HR-ESI-MS) were obtained on a Bruker APEX IV Fourier transform ion cyclotron resonance mass spectrometer at the Analytical Instrumentation Center at Peking University.