IDENTIFICATION OF MOUSE CHROMOSOMES BY QUINACRINE MUSTARD STAINING
IDENTIFICATION OF MOUSE CHROMOSOMES BY QUINACRINE MUSTARD STAINING
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DOI：
10.1159/000130154
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
NESBITT, M
中科院分区：
文献类型：
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作者：
FRANCKE, U;NESBITT, M
用DNA结合荧光染料奎纳克林芥末对各种近交和随机繁殖类型的雄性和雌性小鼠的中期扩散进行染色。这种处理使染色体显示出荧光显微镜下可见的亮带和暗带的横纹图案。荧光强度分布沿着每条染色体被发现是独特的和可重复的，因此，它是可能的，以区分和识别所有的染色体对的小鼠核型。平均而言，X染色体是雌性细胞中第四大对。Y染色体通常是最小的染色体，荧光最强。荧光染色体的密度计示踪导致独特的密度分布，这可以帮助染色体鉴定。
Metaphase spreads from male and female mice of various inbred and random-bred types were stained with the DNA-binding fluorochrome quinacrine mustard. This treatment caused the chromosomes to display cross-striation patterns of bright and dark bands visible with the fluorescence microscope. The intensity distribution of fluorescence along each chromosome was found to be distinctive and reproducible, so that it was possible to differentiate and identify all chromosome pairs in the mouse karyotype. On the average, the X chromosome was the fourth largest pair in female cells. The Y was usually the smallest chromosome and showed the strongest fluorescence. Densitometer tracing of fluorescent chromosomes resulted in distinctive density profiles which could assist in chromosome identification.