Cathepsin B and L activities in isolated osteoclasts.
Cathepsin B and L activities in isolated osteoclasts.
复制标题
分离的破骨细胞中的组织蛋白酶 B 和 L 活性。
DOI：
10.1016/0006-291x(91)91334-9
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发表时间：
1991
影响因子：
3.1
通讯作者：
Zimmerman,M
中科院分区：
文献类型：
--
作者：
Rifkin,BR;Vernillo,AT;Kleckner,AP;Auszmann,JM;Rosenberg,LR;Zimmerman,M
用顺序过滤分离的鸡破骨细胞检测组织蛋白酶B和L的活性，并将抑制剂添加到牛皮质骨上分解的大鼠破骨细胞中。组织蛋白酶L选择性抑制剂z - ph - ph - chn2在1、5和10 μM浓度下对大鼠破骨细胞的骨吸收有50%、85%和100%的抑制作用，对鸡破骨细胞的组织蛋白酶L活性也有明显的抑制作用。鸡破骨细胞中组织蛋白酶L比组织蛋白酶b高25倍。鸡破骨细胞经广义半胱氨酸蛋白酶抑制剂z - ph - ala - chn2处理后，两种组织蛋白酶均受到相同程度的抑制。组织蛋白酶L可能在吸收中起关键作用。
Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 μM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.