A native soluble form of CTLA-4
A native soluble form of CTLA-4
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DOI：
10.1006/cimm.2000.1649
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发表时间：
2000-05-01
影响因子：
4.3
通讯作者：
Tector, AJ
中科院分区：
文献类型：
--
作者：
Oaks, MK;Hallett, KM;Tector, AJ
CTLA-4是在活化的T和B淋巴细胞的表面上表达的免疫调节受体。CTLA-4的反受体是B7家族分子。我们描述了在人类、小鼠和大鼠的血淋巴组织中表达的选择性剪接mRNA，这些mRNA缺乏由CTLA-4基因外显子3编码的跨膜结构域。通过RT-PCR在CD 4和CD 8表型的B细胞和静息T细胞中检测到这些替代转录物。用PHA或抗-CD 3+抗-CD 28单克隆抗体激活人血单核细胞似乎会导致替代转录物相对于全长转录物的量减少。重组sCTLA-4是一种B7结合蛋白，具有免疫调节作用，通过抑制混合白细胞反应来测量。人血清含有与天然可溶形式的CTLA-4一致的免疫反应性物质。(C)北京大学出版社.
CTLA-4 is an immunoregulatory receptor expressed on the surface of activated T and B lymphocytes. The counterreceptors for CTLA-4 are the B7 family molecules. We describe alternatively spliced mRNAs expressed in hematolymphoid tissues of humans, mice, and rats that lack the transmembrane domain coded by exon 3 of the CTLA-4 gene. These alternate transcripts were detected by RT-PCR in B cells and resting T cells of both the CD4 and the CD8 phenotype. Activation of human blood mononuclear cells with PHA or anti-CD3 + anti-CD28 monoclonal antibodies appears to effect a decrease in the amount of the alternative transcript relative to the full-length transcript. Recombinant sCTLA-4 is a B7-binding protein and has immunomodulatory effects as measured by inhibition of the mixed leukocyte response. Human serum contains immunoreactive material consistent with a native soluble form of CTLA-4. (C) 2000 Academic Press.