Ammonium persulfate: a safe alternative oxidizing reagent for measuring urinary iodine.
Ammonium persulfate: a safe alternative oxidizing reagent for measuring urinary iodine.
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DOI：
10.1093/clinchem/42.2.239
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发表时间：
1996-02
影响因子：
9.3
通讯作者：
S. Pino;Shih-Lieh Fang;Lewis E. Braverman
中科院分区：
文献类型：
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作者：
S. Pino;Shih-Lieh Fang;Lewis E. Braverman
氯酸法最常用于获得准确和可重复的碘测量和去除干扰物质。不幸的是，氯酸是一种潜在的危险，需要一个防爆罩，以及其他预防措施。采用1 mol/L的过硫酸铵作为氧化试剂，建立了一种简便、经济的测定尿碘的方法。在91-95摄氏度的温度下，氧化过程可在30分钟内完成，然后通过对传统的Sandell和Kolthoff比色法的改进来测量尿液中的碘。从美国6 - 79岁的健康男女混合人群中收集尿液样本（110份），采用两种方法分析尿碘含量：提议的过硫酸铵法和氯酸法。过硫酸铵法在0.42 +/- 0.04微mol/L（平均+/- SD）条件下的法内CV为9.1%，在1.46 +/- 0.11微mol/L条件下为7.8%，在3.54 +/- 0.14微mol/L条件下为4.0%。在0.46 +/- 0.05微mol/L时，测定间CV值为10.2%；在3.27 +/- 0.26微mol/L时，测定间CV值为7.9%。在0.42微mol/L、0.77微mol/L和3.64微mol/L条件下，体外加碘回收率分别为107%、94%和97%。检测下限为0.0034微克碘。参考氯酸法测定110例尿中碘值为0.06 ~ 8.03微mol/L，过硫酸铵法测定的碘值为0.05 ~ 7.4微mol/L。过硫酸盐法(y)与参比氯酸法(x)的Pearson相关关系非常密切（y = 0.923x + 0.810 micromol/L, r = 0.994, Sy/x = 1.841）。
The chloric acid method is most commonly used to obtain accurate and reproducible measurements of iodine and remove interfering substances. Unfortunately chloric acid is a potential hazard, requiring an explosion-proof hood, among other precautions. We have developed a simple, convenient, and economic method for measuring urinary iodine by using 1 mol/L ammonium persulfate, a nonexplosive, nonhazardous chemical, as the oxidizing reagent. The oxidation procedure can be completed in 30 min at a temperature of 91-95 degrees C. The iodine in the urine is then measured by a modification of the traditional colorimetric method of Sandell and Kolthoff. Urine samples (110) collected from a mixed population of healthy males and females, ranging in age from 6 to 79 years and living in the US, were analyzed for urine iodine content by two methods: the proposed ammonium persulfate method and the chloric acid method. The ammonium persulfate method has an intraassay CV of 9.1% at 0.42 +/- 0.04 micromol/L (mean +/- SD), 7.8% at 1.46 +/- 0.11 micromol/L, and 4.0% at 3.54 +/- 0.14 micromol/L. The interassay CV is 10.2% at 0.46 +/- 0.05 micromol/L, and 7.9% at 3.27 +/- 0.26 micromol/L. Recovery of iodine added to urine in vitro was 107%, 94%, and 97% for 0.42 micromol/L, 0.77 micromol/L and 3.64 micromol/L, respectively. The lower limit of detectability was 0.0034 microgram of iodine. Values for iodine in 110 urines measured by the reference chloric acid method ranged from 0.06 to 8.03 micromol/L and by the ammonium persulfate method from 0.05 to 7.4 micromol/L. The persulfate method (y) correlated extremely closely with the reference chloric acid method (x) by the Pearson correlation (y = 0.923x + 0.810 micromol/L, and r = 0.994, Sy/x = 1.841).