Specific attachment and migration of human astrocytoma cells on human but not murine laminin.
Specific attachment and migration of human astrocytoma cells on human but not murine laminin.
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人类星形细胞瘤细胞在人类而非小鼠层粘连蛋白上的特异性附着和迁移。
DOI：
10.1002/glia.440130108
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Berens,ME
中科院分区：
文献类型：
--
作者：
Giese,A;Rief,MD;Tran,NL;Berens,ME
从小鼠EHS肉瘤中分离的层粘连蛋白的附着位置和生物学功能已经得到了很好的研究。最近，包括人胎盘层粘连蛋白在内的几种变异型层粘连蛋白已被证明与EHS-层粘连蛋白不同。本研究旨在研究人星形细胞瘤细胞系对人和小鼠肉瘤EHS-层粘连蛋白的附着、增殖和迁移现象。在短期附着试验中，人胎盘层粘连蛋白被证明是更好的细胞黏附底物。EHS-层粘连蛋白对人层粘连蛋白的作用约占观察到的30-50%。RT-PCR法检测星形细胞瘤细胞表达β-1、β-3和β-4亚单位。抗β抗体可阻断与EHS-层粘连蛋白的黏附，而抗β1、β4和αv亚基抗体均不能阻断与人层粘连蛋白的黏附。迁移分析表明，人层粘连蛋白上的星形细胞瘤细胞分散在中央播种区，而EHS-层粘连蛋白上的细胞仍留在播种的地方。星形细胞瘤细胞在不同蛋白质上生长速度的变化不能解释这种分散模式，因为两种细胞系在两种层粘连蛋白上生长得同样好。我们的结论是，人层粘连蛋白上的独特表位被人类星形细胞瘤细胞上的新型受体识别，这种受体赋予细胞迁移表型。©1995 Wiley-Liss公司
Attachment sites and biological functions of laminin isolated from murine EHS sarcoma have been well studied. Recently several variants of laminin including human placental laminin have been shown to be distinct from EHS‐laminin. This study was undertaken to determine attachment, proliferation, and migration phenomena of human astrocytoma cell lines to human and murine sarcoma EHS‐laminin. Using short‐term attachment assays human placental laminin was shown to be the better substrate for cell adhesion. EHS‐laminin mediated approximately 30–50% of the effect observed on human laminin. The astrocytoma cells expressed β1, β3, and β4 subunit mRNA as determined by RT‐PCR. Anti‐β antibodies blocked adhesion to EHS‐laminin, but antibodies against β1, β4, and αvsubunits were all ineffective in blocking adhesion to human laminin. A migration assay showed that astrocytoma cells on human laminin dispersed from a central seeding area, while cells on EHS‐laminin remained where they were seeded. The pattern of dispersion could not be accounted for by changes in growth rates of astrocytoma cells on the different proteins, since both cell lines grew equally well on the two laminins. We conclude that unique epitopes on human laminin are recognized by novel receptors on human astrocytoma cells which confer a migratory phenotype to the cells. © 1995 Wiley‐Liss, Inc.