Toward full spectrum speciation of silver nanoparticles and ionic silver by on-line coupling of hollow fiber flow field-flow fractionation and minicolumn concentration with multiple detectors.
Toward full spectrum speciation of silver nanoparticles and ionic silver by on-line coupling of hollow fiber flow field-flow fractionation and minicolumn concentration with multiple detectors.
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DOI：
10.1021/acs.analchem.5b01827
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发表时间：
2015-08
影响因子：
7.4
通讯作者：
Zhiqiang Tan;Jing-fu Liu;Xiaoru Guo;Yong-guang Yin;Seul Kee Byeon;M. Moon;G. Jiang
中科院分区：
文献类型：
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作者：
Zhiqiang Tan;Jing-fu Liu;Xiaoru Guo;Yong-guang Yin;Seul Kee Byeon;M. Moon;G. Jiang
银纳米颗粒（AgNPs）和离子银（Ag（I））在环境中的相互转化决定了它们的运输、摄取和毒性，需要同时分离和定量AgNPs和Ag（I）的方法。首次将中空纤维流场流分级（HF 5）和微柱浓缩与多个检测器（包括紫外-可见光谱法、动态光散射和电感耦合等离子体质谱法）在线耦合在一起，用于各种Ag（I）物质（即，游离Ag（I）、弱和强Ag（I）络合物）和不同尺寸的AgNP。当HF 5用于过滤和分级AgNP（> 2nm）时，填充有Amberlite IR 120树脂的微型柱起到捕获来自HF 5径向流的游离Ag（I）或弱Ag（I）络合物以及强Ag（I）络合物和微小AgNP（90%的微小AgNP与游离Ag（I）相比）的作用，并被捕获在微型柱中。结果表明，该方法对银纳米粒子的表征结果与透射电子显微镜的表征结果吻合较好，且回收率较高（70.7-108%），包括Ag（I）、Ag（I）和半胱氨酸的加合物，以及标称直径为1.4 nm、10 nm、20 nm、40 nm的五种AgNP，在地表水样品中为60 nm。
The intertransformation of silver nanoparticles (AgNPs) and ionic silver (Ag(I)) in the environment determines their transport, uptake, and toxicity, demanding methods to simultaneously separate and quantify AgNPs and Ag(I). For the first time, hollow fiber flow field-flow fractionation (HF5) and minicolumn concentration were on-line coupled together with multiple detectors (including UV-vis spectrometry, dynamic light scattering, and inductively coupled plasma mass spectrometry) for full spectrum separation, characterization, and quantification of various Ag(I) species (i.e., free Ag(I), weak and strong Ag(I) complexes) and differently sized AgNPs. While HF5 was employed for filtration and fractionation of AgNPs (>2 nm), the minicolumn packed with Amberlite IR120 resin functioned to trap free Ag(I) or weak Ag(I) complexes coming from the radial flow of HF5 together with the strong Ag(I) complexes and tiny AgNPs (90% of tiny AgNPs to free Ag(I) and trapped in the minicolumn. The excellent performance was verified by the good agreement of the characterization results of AgNPs determined by this method with that by transmission electron microscopy, and the satisfactory recoveries (70.7-108%) for seven Ag species, including Ag(I), the adduct of Ag(I) and cysteine, and five AgNPs with nominal diameters of 1.4 nm, 10 nm, 20 nm, 40 nm, and 60 nm in surface water samples.