NaCl increases the activity of the plasma membrane H+-ATPase in C3 halophyte Suaeda salsa callus
NaCl increases the activity of the plasma membrane H+-ATPase in C3 halophyte Suaeda salsa callus
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DOI：
10.1007/s11738-009-0371-7
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发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
Wang, Bao-Shan
中科院分区：
文献类型：
--
作者：
Chen, Min;Song, Jie;Wang, Bao-Shan
以盐地碱蓬愈伤组织为材料，研究了质膜H+-ATPase对盐胁迫的响应及其在耐盐性中的作用。适宜愈伤组织生长的氯化钠浓度为50 mM，而250 mM的氯化钠对愈伤组织生长有明显的抑制作用。愈伤组织的离子含量和有机溶质含量均随盐浓度的增加而增加。在一定浓度范围内(0-150 mM的氯化钠)，愈伤组织的质膜H+-ATPase活性增加。而在150 mM的氯化钠作用下，酶活达到最大。用抗玉米H+-ATPase血清(46E5B11D5)对愈伤组织培养的PM H+-ATPase蛋白进行免疫印迹分析，鉴定出一种约90 kDa的单一多肽。在150 mM的氯化钠溶液中，愈伤组织中的多肽水平与对照相比有所增加，但在50 mM的氯化钠溶液中，增加的幅度较小。Northern印迹分析表明，经盐处理的愈伤组织中，质膜H+-ATPase的表达也增加。这些结果表明，盐碱蓬愈伤组织中质膜H+-ATPase活性的增加既是由于质膜H+-ATPase蛋白含量的增加，也是由于PM H+-ATPase基因的上调，后者与盐碱蓬愈伤组织的耐盐性有关。
Suaeda salsa calli treated with different concentrations of NaCl were used to examine the response of the plasma membrane (PM) H+-ATPase to NaCl and its role in salt tolerance. The optimum concentration of NaCl for growth of the calli was 50 mM, while growth was significantly inhibited at 250 mM NaCl. The ion and organic solute contents of calli increased with increasing NaCl. Activity of the PM H+-ATPase increased when the calli were treated with NaCl over a certain concentration range (0-150 mM NaCl). However, the activity reached its maximum with 150 mM NaCl. Immunoblotting analysis of the PM H+-ATPase protein from calli cultures with anti-Zea mays H+-ATPase serum (monoclonal 46E5B11D5) identified a single polypeptide of similar to 90 kDa. The peptide levels increased in the calli treated with NaCl at 150 mM NaCl compared to control, but the increase at 50 mM NaCl was less pronounced. Northern blot analysis showed that the expression of the PM H+-ATPase also increased after the calli were treated with NaCl. These results suggest that the increase in PM H+-ATPase activity is due to both an increase in the amount of PM H+-ATPase protein and an up-regulation of the PM H+-ATPase gene, which is involved in the salt tolerance of S. salsa calli.