Fusobacterium nucleatum supports the growth of Porphyromonas gingivalis in oxygenated and carbon-dioxide-depleted environments
Fusobacterium nucleatum supports the growth of Porphyromonas gingivalis in oxygenated and carbon-dioxide-depleted environments
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DOI：
10.1099/00221287-148-2-467
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发表时间：
2002-02-01
期刊：
影响因子：
2.8
通讯作者：
Rogers, AH
中科院分区：
文献类型：
--
作者：
Diaz, PI;Zilm, PS;Rogers, AH
作者比较了厌氧牙周病细菌具核梭杆菌和牙龈卟啉单胞菌对氧气耐受性的差异，并探讨了具核梭杆菌可能能够支持牙龈卟啉单胞菌在通气和二氧化碳耗尽的环境中生长的可能性。两种微生物均使用连续培养系统在存在和不存在CO2以及不同通气条件下以单一培养物和共培养物的形式生长。在稳定状态下，进行活菌计数并测定牙龈卟啉单胞菌中超氧化物歧化酶和NADH氧化酶/过氧化物酶的活性。在共培养中，具核梭杆菌能够支持牙龈卟啉单胞菌在通气和二氧化碳耗尽的环境中生长，而牙龈卟啉单胞菌作为单一培养物无法在其中生存。具核梭杆菌不仅对氧气的耐受性比牙龈卟啉单胞菌高得多，而且在中等含氧条件下其数量也显着增加。具核梭菌可能在牙菌斑成熟过程中发挥着额外的间接作用，有助于减少牙龈卟啉单胞菌和可能其他耐氧性较差的厌氧菌生存所需的条件。此外，具核梭菌能够产生牙龈卟啉单胞菌生长所必需的嗜二氧化碳环境。
The authors compared the differences in tolerance to oxygen of the anaerobic periodontopathic bacteria Fusobacterium nucleatum and Porphyromonas gingivalis, and explored the possibility that F. nucleatum might be able to support the growth of P. gingivalis in aerated and CO2 -depleted environments. Both micro-organisms were grown as monocultures and in co-culture in the presence and absence of CO2 and under different aerated conditions using a continuous culture system. At steady state, viable counts were performed and the activities of the enzymes superoxide dismutase and NADH oxidase/peroxidase were assayed in P. gingivalis. In co-culture, F. nucleatum was able to support the growth of P. gingivalis in aerated and CO2 -depleted environments in which P. gingivalis, as a monoculture, was not able to survive. F. nucleatum not only appeared to have a much higher tolerance to oxygen than P. gingivalis, but a significant increase in its numbers occurred under moderately oxygenated conditions. F. nucleatum might have an additional indirect role in dental plaque maturation, contributing to the reducing conditions necessary for the survival of P. gingivalis and possibly other anaerobes less tolerant to oxygen. Additionally, F. nucleatum is able to generate a capnophilic environment essential for the growth of P. gingivalis.