Presence of Brown Adipocytes in Retroperitoneal Fat From Patients With Benign Adrenal Tumors: Relationship With Outdoor Temperature
Presence of Brown Adipocytes in Retroperitoneal Fat From Patients With Benign Adrenal Tumors: Relationship With Outdoor Temperature
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DOI：
10.1210/jc.2012-3535
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发表时间：
2013-10-01
影响因子：
5.8
通讯作者：
Enerback, Sven
中科院分区：
文献类型：
--
作者：
Betz, Matthias Johannes;Slawik, Marc;Enerback, Sven
背景：棕色脂肪组织（BAT）是一种代谢高度活跃的器官，在暴露于低温的啮齿动物中具有增加的产热活性。最近，它在人类成人颈部脂肪组织中的存在及其与有利代谢表型的关联已被报道。目的：本研究的目的是确定成人腹膜后BAT的患病率。设计：这是一项观察性队列研究。环境：本研究在一家三级转诊医院进行。患者：本研究包括57例接受良性肾上腺肿瘤手术的患者。主要观察指标：通过解偶联蛋白1 （uncoupling protein 1, UCP1）蛋白和mRNA的表达，测定切除肾上腺肿瘤附近腹膜后BAT的患病率。结果：通过蛋白和mRNA表达分析，57例患者中有26例（45.6%）检测到UCP1蛋白，棕色脂肪细胞特征基因mRNA高表达，与肾上腺肿瘤类型无关。腹膜后脂肪中棕色脂肪细胞的存在与手术前一个月室外温度显著降低有关。重要的是，UCP1 mRNA和蛋白水平的表达与室外温度呈负相关，而体重指数、性别、年龄和糖尿病状态则不相关。结论：这些发现表明人类腹膜后脂肪组织可以获得BAT表型，从而适应环境挑战。这些适应性过程可能为肥胖和胰岛素抵抗的治疗提供有价值的治疗靶点。
Context: Brown adipose tissue (BAT) is a metabolically highly active organ with increased thermogenic activity in rodents exposed to cold temperature. Recently its presence in the cervical adipose tissue of human adults and its association with a favorable metabolic phenotype have been reported.Objective: The objective of the study was to determine the prevalence of retroperitoneal BAT in human adults.Design: This was an observational cohort study.Setting: The study was conducted at a tertiary referral hospital.Patients: Fifty-seven patients who underwent surgery for benign adrenal tumors were included in this study.Main Outcome Measures: Prevalence of retroperitoneal BAT adjacent to the removed adrenal tumor as determined by uncoupling protein 1 (UCP1) protein and mRNA expression was measured.Results: Using protein and mRNA expression analysis, we detected UCP1 protein in 26 of 57 patients (45.6%) as well as high mRNA expression of genes characteristic for brown adipocytes, independent of the adrenal tumor type. The presence of brown adipocytes within the retroperitoneal fat was associated with a significantly lower outdoor temperature during the month prior to surgery. Importantly, UCP1 expression on both mRNA and protein level was inversely correlated to outdoor temperature, whereas body mass index, sex, age, and diabetes status were not.Conclusions: These findings suggest that human retroperitoneal adipose tissue can acquire a BAT phenotype, thereby adapting to environmental challenges. These adaptive processes might provide a valuable therapeutic target in the treatment of obesity and insulin resistance.