CYTOPATHOGENIC EFFECT OF BRUCELLA SPHEROPLASTS ON MONOCYTES IN TISSUE CULTURE
CYTOPATHOGENIC EFFECT OF BRUCELLA SPHEROPLASTS ON MONOCYTES IN TISSUE CULTURE
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DOI：
10.1128/jb.88.5.1310-1315.1964
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发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
RUMACK, BH
中科院分区：
文献类型：
--
作者：
FREEMAN, BA;RUMACK, BH
来自豚鼠腹膜渗出液的单核吞噬细胞被证明既能摄取正常的猪布鲁氏菌，也能摄取经甘氨酸和青霉素处理的猪布鲁氏菌制备的球质体。对p32标记的布鲁氏菌的定量摄入研究表明，粗糙的正常布鲁氏菌的摄入率高于光滑的正常布鲁氏菌。光滑细胞制备的球质体的吞噬率高于正常光滑细胞，粗糙布鲁氏菌制备的球质体的吞噬率较高，但明显低于正常粗糙布鲁氏菌制备的球质体。所有球质体的吞噬程度都呈现出先达到峰值后下降的趋势，表明所摄入的细菌被释放；这种胞内细菌的释放被认为是由于球质体发挥的细胞致病性作用。直接显微镜观察表明，用光滑或粗糙的布鲁氏菌制备的活球质体感染后，在4小时内破坏了宿主细胞的大部分，但福尔马林杀死的球质体并不比正常的布鲁氏菌更具破坏性。当通过释放细胞成分到培养基中来检测宿主细胞破坏时，很明显，球质体对宿主细胞的破坏在摄入开始后0.5小时内达到显著水平，并在4小时内几乎完成。
Mononuclear phagocytes from guinea pig peritoneal exudates were shown to ingest both normal Brucella suis and spheroplasts prepared from B. suis by treatment with glycine and with penicillin. Quantitative ingestion studies with P32-labeled Brucella showed that rough normal Brucella are ingested at a greater rate than are smooth normal Brucella. Spheroplasts prepared from smooth cells were phagocytized at a greater rate than were the normal smooth cells, and spheroplasts prepared from rough Brucella were phagocytized well, although apparently to a lesser extent than from the normal rough Brucella. The degree of phagocytosis of all spheroplasts appeared to reach a peak and then decrease, indicating a release of ingested bacteria; this release of intracellular bacteria is believed to be due to the cytopathogenic effect exerted by the spheroplasts. Direct microscopic observations showed that infection with living spheroplasts prepared from either smooth or rough Brucella destroyed a major portion of the host cells within 4 hr, but that formalin-killed sphero- plasts were no more destructive than were normal Brucella. When host cell destruction was assayed by the release of cellular constituents into the medium, it was apparent that host-cell destruction by spheroplasts reached a significant level within 0.5 hr after ingestion begins, and is almost complete by 4 hr.