Highly active and stable DNAzyme - Carbon nanotube hybrids
Highly active and stable DNAzyme - Carbon nanotube hybrids
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DOI：
10.1021/ja0541581
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发表时间：
2005-09-07
影响因子：
15
通讯作者：
Dordick, JS
中科院分区：
文献类型：
--
作者：
Yim, TJ;Liu, JW;Dordick, JS
在此，我们报道了脱氧核酶−碳纳米管共轭化合物的生成和表征。将生物素化的DNA酶连接到链霉亲和素功能化的多壁碳纳米管上。得到的脱氧核酶−多壁碳纳米管结合物符合米氏−动力学和多重催化转化率。所合成的纳米偶联物的比活力为自由酶的∼/3。如此高的活性可能允许无数的应用，从纳米材料的定向组装到依赖于DNAzyme的RNA裂解能力的纳米级细胞疗法。
We report herein the generation and characterization of DNAzyme−carbon nanotube conjugates. Biotinylated DNAzyme was attached to streptavidin-functionalized MWNTs. The resultant DNAzyme−MWNT conjugates exhibited Michaelis−Menten kinetics and multiple catalytic turnovers. The specific activity of the nanoscale conjugates was ∼/3of that for freely soluble DNAzyme. Such high activity may allow myriad applications ranging from the directed assembly of nanomaterials to nanoscale cellular therapeutics that rely on the RNA cleaving ability of DNAzymes.