20(S)-protopanaxatriol, one of ginsenoside metabolites, inhibits inducible nitric oxide synthase and cyclooxygenase-2 expressions through inactivation of nuclear factor-κB in RAW 264.7 macrophages stimulated with lipopolysaccharide
20(S)-protopanaxatriol, one of ginsenoside metabolites, inhibits inducible nitric oxide synthase and cyclooxygenase-2 expressions through inactivation of nuclear factor-κB in RAW 264.7 macrophages stimulated with lipopolysaccharide
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DOI：
10.1016/j.canlet.2003.09.037
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发表时间：
2004-03-08
期刊：
影响因子：
9.7
通讯作者：
Chung, HT
中科院分区：
文献类型：
--
作者：
Oh, GS;Pae, HO;Chung, HT
人参中的人参皂苷在口服人参提取物后被人体肠道细菌代谢。20(S)-原人参三醇(PPT)是人参皂苷的主要代谢产物之一。诱导型一氧化氮合酶(NOS)和环氧合酶-2(COX-2)是介导炎症过程的重要酶。INOS和/或COX-2的不适当上调与炎症性疾病和某些类型的人类癌症的发病机制有关。在这里，我们研究了PPT是否能调节内毒素刺激的RAW 264.7巨噬细胞中一氧化氮合酶和环氧合酶-2的表达。我们发现，PPT通过抑制I-kappaBalpha的磷酸化和降解，使核因子-kappaB失活，从而阻断了内毒素诱导的iNOS和COX-2表达的增加。因此，有可能开发PPT作为一种有用的化学预防癌症或炎症性疾病的药物。(C)2003爱思唯尔爱尔兰有限公司。保留所有权利。
Ginsenosides from Panax ginseng are metabolized by human intestinal bacteria after oral administration of ginseng extract. 20(S)-Protopanaxatriol (PPT) is one of the major metabolites of ginsenosides. Inducible nitric oxide synthase (NOS) and cyclooxygenase-2 (COX-2) are important enzymes that mediate inflammatory processes. Improper up-regulation of iNOS and/or COX-2 has been associated with the pathogenesis of inflammatory diseases and certain types of human cancers. Here, we investigated whether PPT could modulate NOS and COX-2 expressions in RAW 264.7 macrophages stimulated with the endotoxin lipopolysaccharide (LPS). We found that PPT blocked the increase in LPS-induced iNOS and COX-2 expressions through inactivation of nuclear factor-kappaB by preventing I-kappaBalpha phosphorylation and degradation. Thus, it may be possible to develop PPT as a useful agent for chemoprevention of cancer or inflammatory diseases. (C) 2003 Elsevier Ireland Ltd. All rights reserved.