Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels
Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels
复制标题
DOI：
10.1002/jbm.a.20010
复制
发表时间：
2004-02-01
影响因子：
4.9
通讯作者：
Stürmer, KM
中科院分区：
文献类型：
--
作者：
Frosch, KH;Barvencik, F;Stürmer, KM
本研究的目的是探讨不同直径的圆柱形钛通道对人成骨细胞的影响。将直径分别为300,400,500,600和1000mm的连续钻孔钛样品放入从12名成人创伤患者分离的成骨细胞培养物中。通过透射光显微镜观察细胞向孔道的迁移。采用分光光度法测定钻孔通道中DNA含量，酶联免疫吸附法（ELISA）检测ⅰ型胶原蛋白的产生，逆转录聚合酶链反应（RT-PCR）检测骨钙素基因的表达。通过组织切片的显微x线摄影评估矿化组织的形成。20 d内，细胞在直径为600 μ m的孔道中平均生长838 μ m (+/-128 μ m)，显著快于其他孔道（p < 0.05）。细胞在600 μ m通道中产生的骨钙素信使RNA （mRNA）显著高于1000 μ m通道（p < 0.05），并表现出最高的成骨分化。通道直径不影响I型胶原蛋白的产生。300 μ m通道细胞密度最高（p < 0.05）。随着通道直径的增加，通道的DNA含量呈线性下降。培养40 d后，300 ~ 600 μ m孔口部矿化组织比例为6%，400 ~ 600 μ m孔口部矿化组织比例为9 ~ 11%。在1000 μ m的河道中，仅检测到矿化痕迹。我们的数据表明，圆柱形钛通道的直径对人成骨细胞的迁移、基因表达和矿化有显著影响。(C) 2003 Wiley期刊有限公司
The purpose of the current study was to investigate the effect of different diameters of cylindrical titanium channels on human osteoblasts. Titanium samples having continuous drill channels with diameters of 300, 400, 500, 600, and 1000 mum were put into osteoblast cell cultures that were isolated from 12 adult human trauma patients. Cell migration into the drill channels was investigated by transmitted-light microscopy. The DNA content in the drill channels was measured photometrically, collagen type I production was analyzed by enzyme-linked immunosorbent assay (ELISA) and osteocalcin gene expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Formation of mineralized tissue was assessed by microradiographs of histological sections. Within 20 days, cells grew an average of 838 mum (+/-128 mum) into the drill channels with a diameter of 600 mum and were significantly faster (p < 0.05) than in all other channels. Cells produced significantly more osteocalcin messenger RNA (mRNA) in 600-mum channels (p < 0.05) than they did in 1000-mum channels and demonstrated the highest osteogenic differentiation. The channel diameter did not influence collagen type I production. The highest cell density was found in 300-mum channels (p < 0.05). The DNA content of the channels linearly decreased with increasing channel diameters. After 40 days of culture, the proportion of mineralized tissue at the mouth section amounted to 6% in 300-mum channels and to 9-11% in 400-600-mum channels. In 1000-mum channels, only traces of mineralization were detected. Our data suggest that the diameter of cylindrical titanium channels has a significant effect on migration, gene expression, and mineralization of human osteoblasts. (C) 2003 Wiley Periodicals, Inc.