The contribution of TNF-α in the amygdala to anxiety in mice with persistent inflammatory pain
The contribution of TNF-α in the amygdala to anxiety in mice with persistent inflammatory pain
复制标题
DOI：
10.1016/j.neulet.2013.02.005
复制
发表时间：
2013-04-29
影响因子：
2.5
通讯作者：
Su, Hai-chuan
中科院分区：
文献类型：
--
作者：
Chen, Jun;Song, Yang;Su, Hai-chuan
肿瘤坏死因子α（TNF-α）以其在炎症和疼痛中的作用而闻名，炎症和疼痛与焦虑和抑郁等情绪障碍密切相关。杏仁核是调节焦虑的前脑结构。然而，人们对TNF-α在慢性疼痛动物焦虑发展中的作用知之甚少。在本研究中，我们研究了在小鼠后爪注射完全弗氏佐剂（CFA）诱导炎症后，基底外侧杏仁核（BLA）中TNF-α的表达。我们还确定了TNF-α表达对这些小鼠焦虑发展的影响。在CFA诱导的外周炎症慢性期，BLA中TNF-α表达增加。在BLA中局部输注TNF-α中和抗体英夫利西单抗逆转了持续性炎性疼痛小鼠的焦虑样行为。体外切片记录显示，TNF-α显著增强BLA中AMPA受体介导的兴奋性突触传递，并抑制GABA（A）受体介导的抑制性突触传递。因此，我们的研究结果提供了强有力的证据，表明TNF-α有助于持续性炎性疼痛小鼠的焦虑发展。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Tumor necrosis factor alpha (TNF-alpha) is known for its role in inflammation and pain, which are strongly associated with mood disorders such as anxiety and depression. The amygdala is a forebrain structure that modulates anxiety. However, little is known about the role of TNF-alpha in the development of anxiety in animals with chronic pain. In the present study, we examined TNF-alpha expression in the basolateral amygdala (BLA) following injection of complete Freund's adjuvant (CFA) into the hind paw of mice to induce inflammation. We also determined the effects of TNF-alpha expression on the development of anxiety in these mice. TNF-alpha expression was increased in the BLA during the chronic phase of CFA-induced peripheral inflammation. The local infusion of TNF-alpha-neutralizing antibody infliximab in the BLA reversed anxiety-like behaviors in mice with persistent inflammatory pain. In vitro slice recordings revealed that TNF-alpha significantly enhanced AMPA-receptor-mediated glutamatergic excitatory synaptic transmission and inhibited GABA(A)-receptor-mediated inhibitory synaptic transmission in the BLA. Our findings, therefore, provide strong evidence that TNF-alpha contributes to the development of anxiety in mice with persistent inflammatory pain. (C) 2013 Elsevier Ireland Ltd. All rights reserved.