NITRIC-OXIDE SYNTHASE AND NEURONAL NADPH DIAPHORASE ARE IDENTICAL IN BRAIN AND PERIPHERAL-TISSUES
NITRIC-OXIDE SYNTHASE AND NEURONAL NADPH DIAPHORASE ARE IDENTICAL IN BRAIN AND PERIPHERAL-TISSUES
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DOI：
10.1073/pnas.88.17.7797
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
DAWSON, TM;BREDT, DS;SNYDER, SH
NADPH黄递酶染色的神经元对毒性损伤和神经退行性疾病具有独特的抵抗力，它与大脑和周围组织中含有一氧化氮合酶(EC 1.14.23.-)的神经元共存，一氧化氮合酶产生一氧化氮(NO)，这是一种新近发现的神经元信使分子。在纹状体和大脑皮质，一氧化氮合酶免疫反应和NADPH黄递酶染色共存于中到大型的脊髓神经元。这些神经元与生长抑素和神经肽Y免疫反应共同定位。NO合成酶免疫反应和NADPH黄递酶染色均与胆碱乙酰转移酶阳性细胞共存于桥脑脚核，内核层无长突细胞、神经节细胞或视网膜、肠肌间神经丛神经元和肾上腺髓质神经节细胞也共存。不含合酶基因的人肾细胞可见NADPH黄递酶染色。NO合成酶与NADPH黄递酶染色的比值与神经元相同，表明NO合成酶完全解释了观察到的NADPH染色。神经元型NO合成酶和NADPH黄递酶的同一性提示NO在调节神经毒性中起作用。
NADPH diaphorase staining neurons, uniquely resistant to toxic insults and neurodegenerative disorders, have been colocalized with neurons in the brain and peripheral tissue containing nitric oxide synthase (EC 1.14.23.-), which generates nitric oxide (NO), a recently identified neuronal messenger molecule. In the corpus striatum and cerebral cortex, NO synthase immunoreactivity and NADPH diaphorase staining are colocalized in medium to large aspiny neurons. These same neurons colocalize with somatostatin and neuropeptide Y immunoreactivity. NO synthase immunoreactivity and NADPH diaphorase staining are colocalized in the pedunculopontine nucleus with choline acetyltransferase-containing cells and are also colocalized in amacrine cells of the inner nuclear layer and ganglion cells or the retina, myenteric plexus neurons of the intestine, and ganglion cells of the adrenal medulla. Transfection or human kidney cells with NO synthase cDNA elicits NADPH diaphorase staining. The ratio of NO synthase to NADPH diaphorase staining in the transfected cells is the same as in neurons, indicating that NO synthase fully accounts for observed NADPH staining. The identity of neuronal NO synthase and NADPH diaphorase suggests a role for NO in modulating neurotoxicity.