Stimulation by dolichol phosphate-mannose of N-acetylglucosaminyl-lipid biosynthesis by membranes from class E Thy-1-negative mutant mouse lymphoma cells which are defective in dolichol phosphate-mannose biosynthesis.
Stimulation by dolichol phosphate-mannose of N-acetylglucosaminyl-lipid biosynthesis by membranes from class E Thy-1-negative mutant mouse lymphoma cells which are defective in dolichol phosphate-mannose biosynthesis.
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通过来自 E 类 Thy-1 阴性突变型小鼠淋巴瘤细胞的膜，多甘醇磷酸-甘露糖刺激 N-乙酰葡糖胺基-脂质生物合成，这些细胞在多甘醇磷酸-甘露糖生物合成方面存在缺陷。
DOI：
10.1016/0003-9861(86)90711-3
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发表时间：
1986
影响因子：
3.9
通讯作者：
DeBrakeleer,DJ
中科院分区：
文献类型：
--
作者：
Kean,EL;DeBrakeleer,DJ
先前已证明多醇磷酸-甘露糖可刺激 N-乙酰氨基葡萄糖基-二磷酸-多醇的生物合成（E. L. Kean (1985)J. Biol. Chem.260, 12561–12571）。尽管 E 类 Thy-1 阴性突变小鼠淋巴瘤细胞无法合成多甘醇磷酸甘露糖，但将该化合物外源添加到突变细胞膜上会刺激 N-乙酰氨基葡萄糖脂质合成，类似于使用野生型细胞膜获得的刺激。突变细胞保留的这种活性支持了多甘醇磷酸-甘露糖作为葡糖胺基转移酶的内在特性的调节作用，其催化多甘醇途径的初始反应。
Dolichol phosphate-mannose has been shown previously to stimulate the biosynthesis ofN-acetylglucosaminyl-diphosphate-dolichol (E. L. Kean (1985)J. Biol. Chem.260, 12561–12571). Although the class E Thy-1-negative mutant mouse lymphoma cells are unable to synthesize dolichol phosphate-mannose, the addition of this compound exogenously to membranes from the mutant cells brought about a stimulation ofN-acetylglucosaminyl-lipid synthesis similar to that obtained with membranes from wild type cells. The retention of this activity by the mutant cells supports the suggestion of a regulatory role for dolichol phosphate-mannose as an intrinsic property of the glucosaminyltransferase which catalyzes the initial reaction of the dolichol pathway.