Genetic diversity and biogeography of native and introduced populations of Ulva pertusa (Ulvales, Chlorophyta)
Genetic diversity and biogeography of native and introduced populations of Ulva pertusa (Ulvales, Chlorophyta)
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DOI：
10.1111/pre.12123
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发表时间：
2016-04
影响因子：
1.5
通讯作者：
T. Hanyuda;S. Heesch;W. Nelson;J. Sutherland;S. Arai;S. Boo;H. Kawai
中科院分区：
文献类型：
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作者：
T. Hanyuda;S. Heesch;W. Nelson;J. Sutherland;S. Arai;S. Boo;H. Kawai
利用叶绿体、线粒体和细胞核非编码区序列的遗传标记，研究了佩尔图萨（Ulva pertusa, Ulvales，吊兰组）本地和引种居群的遗传多样性。在对该物种进行遗传鉴定以供进一步分析的初步调查中，在包括热带海岸在内的更广泛的采集点中，只在温带海岸发现了灰杉，这表明它是一种温带物种，基本上不分布于热带地区。对于叶绿体和线粒体序列，利用短串联重复序列的重复模式和核苷酸替换来识别单倍型（遗传型）。在假定的原生地（东北亚）和引进种群（北美、澳大利亚、新西兰、智利和欧洲）收集的244个标本中，共鉴定出48个基于叶绿体和线粒体单倍型组合的单倍型。其中，在东北亚地区发现了46个单倍型（H1-H8和H11-H48），而在其他地区只发现了1-5个单倍型。核微卫星序列分析。测定了234份标本的PCR产物长度，包括核微卫星区，共鉴定出17种基因型。其中，在东北亚地区发现了14个基因型，在其他地区发现了1-7个基因型。研究结果支持了该物种原生地在东北亚的假设，并认为该原生地以外的种群为非原生地种群。
Genetic diversity of native and introduced populations of Ulva pertusa (Ulvales, Chlorophyta) was examined using genetic markers of chloroplast, mitochondria and nuclear non‐coding region sequences. In the preliminary investigations to genetically identify the species for further analyses, U. pertusa was found only from temperate coasts of the more extensive collection sites including tropical coasts suggesting that it is a temperate species and basically not distributed in tropical regions. For chloroplast and mitochondrial sequences, repeating patterns of short tandem repeat sequences and nucleotide substitutions were used to recognize the haplotypes (genetic types). A total of 48 haplotypes based on combinations of chloroplast and mitochondrial haplotypes were recognized in the 244 specimens collected in the presumptive native range (Northeast Asia) and introduced populations (North America, Australia, New Zealand, Chile and Europe). Among them, 46 haplotypes (H1–H8 and H11–H48) were recognized in Northeast Asia, whereas only 1–5 haplotypes were recognized in the other areas. Nuclear microsatellite sequences were also analyzed. The lengths of the PCR products including the nuclear microsatellite region of 234 specimens were determined, and a total of 17 genotypes were recognized. Among them, 14 genotypes were found in Northeast Asia, whereas 1–7 genotypes were recognized in the other areas. Based on the results, the hypothesis that the native range of the species is in Northeast Asia was supported, and the populations outside this range were concluded to be non‐indigenous populations.