Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430
Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430
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DOI：
10.1007/s00122-022-04185-x
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发表时间：
2022-08-02
影响因子：
5.4
通讯作者：
Zheng, Qi
中科院分区：
文献类型：
--
作者：
Jia, Hongwei;Feng, Hang;Zheng, Qi
小偃7430是一个重要的部分双倍体，它被用来搭建一座桥梁，以转移有价值的基因从Thinopyrum ponticum（Podp.）Barkworth & D.R.把杜威的理论转化为普通小麦。为了加快这些有用基因在染色体工程丰富栽培小麦遗传变异中的应用，以小偃7430为材料，建立了一套完整的附加系。利用基因组原位杂交和荧光原位杂交（mc-FISH）相结合的方法对各品系的染色体组成进行了分析，并利用小麦SNP微阵列分析确定了各外源染色体的同源性。在苗期和灌浆期（Zadoks等级为z.11和z.73）对具有第6组染色体的异附加系WTA 55进行了抗条锈病鉴定。诊断标记分析表明，它可能携带一个新的抗条锈基因。ponticum。此外，基于特异位点扩增片段测序（SLAF-seq），开发了一种特异于外源染色体的FISH探针和45个分子标记。其中有27个标记分别位于单个外源染色体上，部分标记可用于易位系的鉴定。这套附加系以及分子标记和FISH探针将促进来自Th的丰富变异的渐渗。在小麦改良计划中将ponticum转化为小麦。
Xiaoyan 7430 is a significant partial amphiploid, which is used to set up a bridge for transferring valuable genes from Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey into common wheat. To accelerate the application of these useful genes in enriching the genetic variability of cultivated wheat by chromosome engineering, a complete set of derived addition lines has been created from Xiaoyan 7430. The chromosome composition of each line was characterized by the combination of genomic in situ hybridization and multicolor fluorescence in situ hybridization (mc-FISH), and the homoeology of each alien chromosome was determined by wheat SNP microarray analysis. Addition line WTA55 with alien group-6 chromosome was evaluated resistant to stripe rust isolates at both the seedling and grain-filling stages (Zadoks scale at z.11 and z.73). Diagnostic marker analysis proved that it could carry a novel stripe rust resistance gene derived from Th. ponticum. Furthermore, a FISH probe and 45 molecular markers specific for alien chromosomes were developed based on specific-locus amplified fragment sequencing (SLAF-seq). Of which 27 markers were separately located on single alien chromosome, and some of them could be used to identify the derived translocation lines. This set of addition lines as well as the molecular markers and the FISH probe will promote the introgression of abundant variation from Th. ponticum into wheat in wheat improvement programs.