Hard tissue-forming potential of stem/progenitor cells in human dental follicle and dental papilla
Hard tissue-forming potential of stem/progenitor cells in human dental follicle and dental papilla
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DOI：
10.1016/j.archoralbio.2009.10.011
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Kirita, Tadaaki
中科院分区：
文献类型：
--
作者：
Yagyuu, Takahiro;Ikeda, Etsuko;Kirita, Tadaaki
目的：已有研究表明牙齿组织中存在干/祖细胞，但其在人类牙胚中的特性尚不清楚。本研究的目的是研究牙冠形成期牙囊和牙乳头中的这些细胞，并比较它们在硬组织形成中的潜能。设计：在牙冠形成期使用牙囊细胞和牙乳头细胞，比较它们的增殖能力、细胞表面抗原和在体内外形成硬组织的能力。结果：牙囊细胞和牙乳头细胞具有广泛的增殖能力，表达相似的细胞表面抗原，能够在体内和体外形成硬组织。然而，DFC和DPC之间有两个不同之处。首先，DPC的钙积累量显著高于DFC。结论：我们认为牙冠形成期的牙囊和牙乳头中含有不同类型的干/祖细胞，可能具有在起源特定的谱系方向上的硬组织形成能力。(C)2009爱思唯尔有限公司。保留所有权利。
Objective: The existence of stem/progenitor cells in dental tissue has been suggested but their characterization in the human tooth germ remains elusive. The purpose of this study was to investigate these cells in human dental follicles and dental papillae at the crown-forming stage and compare their potential for hard tissue formation.Design: We used dental follicle cells (DFCs) and dental papilla cells (DPCs) derived from dental follicles and dental papillae at the crown-forming stage and compared their proliferative capacity, cell surface antigens and ability to form hard tissue in vitro and in vivo.Results: Both DFCs and DPCs had extensive proliferation ability, expressed similar cell surface antigens and were capable of forming hard tissue in vivo as well as in vitro. However, there were two differences between DFCs and DPCs. First, DPCs had a significantly higher calcium accumulation than that in DFCs. Second, DFCs expressed a cementoblast marker, whereas DPCs expressed an odontoblast marker.Conclusions: We propose that dental follicles and dental papillae at the crown-forming stage contain different types of stem/progenitor cells and may have hard tissue-forming ability in a possibly origin-specific lineage direction. (C) 2009 Elsevier Ltd. All rights reserved.