Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli
Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli
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DOI：
10.1039/c8lc01397g
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发表时间：
2019-04-21
期刊：
影响因子：
6.1
通讯作者：
Krauss, Thomas F.
中科院分区：
文献类型：
--
作者：
Pitruzzello, Giampaolo;Thorpe, Stephen;Krauss, Thomas F.
迫切需要开发新的方法来及时评估细菌对抗生素的反应。抗生素传统上通过其对培养基中细菌的作用进行评估，这需要24-48小时，并且仅利用单一参数，即生长。在这里，我们提出了一种单细胞水平的多参数方法，从加标培养物到正确分类敏感和耐药菌株大约需要一个小时。通过流体动力学捕获数百种细菌，我们同时监测给药后单个细菌的运动性和形态学的演变。我们展示了这种组合检测方法如何在抗菌剂作用开始时提供对抗菌剂活性的洞察，而这是单一参数和传统测试无法提供的。我们的观察补充了目前基于生长的方法，并强调了未来抗生素敏感性试验需要考虑多个参数。
There is an urgent need to develop novel methods for assessing the response of bacteria to antibiotics in a timely manner. Antibiotics are traditionally assessed via their effect on bacteria in a culture medium, which takes 24-48 h and exploits only a single parameter, i.e. growth. Here, we present a multiparameter approach at the single-cell level that takes approximately an hour from spiking the culture to correctly classify susceptible and resistant strains. By hydrodynamically trapping hundreds of bacteria, we simultaneously monitor the evolution of motility and morphology of individual bacteria upon drug administration. We show how this combined detection method provides insights into the activity of antimicrobials at the onset of their action which single parameter and traditional tests cannot offer. Our observations complement the current growth-based methods and highlight the need for future antimicrobial susceptibility tests to take multiple parameters into account.