Expression of insulin‐like growth factor I messenger ribonucleic acid in regenerating bone after fracture: Influence of indomethacin
Expression of insulin‐like growth factor I messenger ribonucleic acid in regenerating bone after fracture: Influence of indomethacin
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DOI：
10.1002/jbmr.5650070212
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发表时间：
1992-02
影响因子：
6.2
通讯作者：
D. Edwall;P. Prisell;A. Levinovitz;E. Jennische;G. Norstedt
中科院分区：
文献类型：
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作者：
D. Edwall;P. Prisell;A. Levinovitz;E. Jennische;G. Norstedt
对大鼠胫骨骨折后骨痂形成过程中胰岛素样生长因子I(IGF-I)的表达进行了研究。骨痂中IGF-I基因的表达水平在骨折后第8天达到峰值，显示出与对照骨相比诱导的10-15倍。在骨折邻近的胫前肌中，IGF-I的表达水平也有升高的趋势。骨折后6d和8d，在软骨细胞、成骨细胞和肌细胞中可见IGF-I免疫反应阳性。切除垂体的动物与对照组动物的IGF-I免疫反应性无明显差异。应用消炎药消炎痛可降低胫骨骨折模型中IGF-I的mRNA表达。以前的研究结果表明，在活体肌肉再生过程中，IGF-I被激活，在这个模型中，吲哚美辛的应用也减少了IGF-I的表达。这一发现可能表明，炎症反应可能对组织再生过程中IGF-I的激活起重要作用。
Expression of insulin‐like growth factor I (IGF‐I) was studied during time in the callus formed after tibial fracture in rats. Levels of IGF‐I mRNA in callus peaked on the day 8 postfracture, showing a 10‐ to 15‐fold induction compared to control bone. Levels of IGF‐I mRNA tended also to be increased in the fracture‐adjacent musculus tibialis anterior. IGF‐I immunoreactivity was found in cartilaginous cells, osteoblasts, and myocytes 6 and 8 days after fracture. No obvious differences were found between hypophysectomized animals and control animals with regard to IGF‐I immunoreactivity. Administration of the antiinflammatory drug indomethacin decreased the IGF‐I mRNA expression in the tibial fracture model. Previous findings have shown that IGF‐I is activated during in vivo muscle regeneration, and also in this model indomethacin administration reduces the expression of IGF‐I. The finding that indomethacin administration reduces IGF‐I expression could indicate that an inflammatory response may be important for activation of IGF‐I during tissue regeneration.