Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic DNA library construction and RFLP analysis in common wheat.
Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic DNA library construction and RFLP analysis in common wheat.
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小麦的限制性片段长度多态性（RFLP）分析。
DOI：
10.1266/jjg.65.367
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Tsunewaki
中科院分区：
文献类型：
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作者：
Yao;Naoki Mori;K. Tsunewaki
为了构建详细的小麦染色体限制性片段长度多态(RFLP)位点连锁图谱，有必要构建基因组DNA文库并对获得的克隆进行鉴定。49%的克隆具有单一拷贝数或低拷贝数。以91个克隆为探针，用2~4个限制性内切酶对8个普通小麦和1个二粒小麦进行了限制性内切酶分析。约20%的探针和13%的探针-酶组合在普通小麦中表现出遗传多样性。DNA缺失是这些小麦基因组之间遗传差异的主要原因。根据RFLP数据，估算了9个多倍体小麦之间的系统发育距离，并构建了反映它们之间亲缘关系的系统树。
To develop detailed linkage maps of restriction fragment length polymorphism (RFLP) sites in wheat chromosomes, it was necessary to construct a genomic DNA library and to characterize the clones obtained. Forty-nine per cent of the clones were of single or low copy number per genome. With 91 clones of this class, as probes, and with two to four restriction endonucleases, for DNA digestion, RFLPs were examined among eight common wheats and a single emmer wheat. About 20% of the probes, and 13% of the probe-enzyme combinations revealed genetic polymorphism among the common wheats. DNA deletions account for most of the genetic differences among these wheat genomes. Based on the RFLP data, phylogenetic distances among the nine polyploid wheats were estimated, and a dendrogram showing the genetic relationships among them was constructed.