Effect of Hepatic Artery Spasm on a Rat Model of Hepatic Ischemia-Reperfusion Injury
Effect of Hepatic Artery Spasm on a Rat Model of Hepatic Ischemia-Reperfusion Injury
复制标题
肝动脉痉挛对大鼠肝缺血再灌注损伤模型的影响
DOI：
10.1002/jum.14726
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发表时间：
2019-03-01
影响因子：
2.3
通讯作者：
Zhang, Ying-cai
中科院分区：
文献类型：
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作者：
Tang, Hui;Qu, En-ze;Zhang, Ying-cai
目的通过超声观察大鼠肝缺血再灌注损伤（IRI）后肝动脉血流动力学的变化，探讨酚妥拉明（PHT）预处理对肝缺血再灌注损伤的保护作用。方法50只大鼠随机分为3组：假手术组（n = 10）、缺血再灌注对照组（n = 20）和PHT预处理组（n = 20）。各组于再灌注后30分钟（n = 10）和90分钟（n = 10）行彩色多普勒血流显像和超声增强检查。取血分析血清丙氨酸转氨酶和天冬氨酸转氨酶水平，取肝组织标本进行病理分析。结果采用US法，我们发现对照组肝动脉再灌注后30min阻力高于假手术组（平均阻力指数[RI] +/- SD， 0.65 +/- 0.09比0.50 +/- 0.09,P < 0.01），对照组再灌注后30min阻力高于假手术组（RI, 0.65 +/- 0.09比0.50 +/- 0.08,P < 0.01）。但再灌注后30min， PHT组肝动脉阻力和肝脏微循环均优于对照组（RI, 0.54 +/- 0.09 vs 0.65 +/- 0.09, P < 0.05；峰值时间，31.94 +/- 2.02 vs 48.34 +/- 4.74秒，P < 0.01）。与对照组相比，PHT组在再灌注30min时，天冬氨酸转氨酶和丙氨酸转氨酶水平显著降低（P < 0.05）。病理检查显示对照组肝动脉直径较小，血管壁凹陷。结论肝缺血再灌注过程中肝动脉可发生短暂性痉挛，加重肝损伤。酚妥拉明治疗可减轻肝动脉痉挛，改善肝脏灌注，通过改善肝脏微循环减轻肝损伤。
Objectives To investigate hemodynamic changes in the hepatic artery after hepatic ischemia-reperfusion injury (IRI) in rats via ultrasound (US) imaging and to discuss the protective effect of phentolamine (PHT) pretreatment on hepatic IRI. Methods Fifty rats were randomly divided into 3 groups: a sham operation group (n = 10), a control ischemia-reperfusion group (n = 20), and a PHT pretreatment group (n = 20). Color Doppler flow imaging and contrast-enhanced US examinations were performed in each group at 30 minutes (n = 10) and 90 minutes (n = 10) after reperfusion. Blood samples were obtained to analyze serum alanine aminotransferase and aspartate aminotransferase levels, and liver tissue specimens were collected for pathologic analysis. Results Using US, we found that hepatic artery resistance at 30 minutes after reperfusion in the control group was higher than that in the sham group (mean resistive index [RI] +/- SD, 0.65 +/- 0.09 versus 0.50 +/- 0.09; P < .01), which was higher at 30 than 90 minutes (RI, 0.65 +/- 0.09 versus 0.50 +/- 0.08; P < .01) after reperfusion in the control group. However, the hepatic artery resistance and liver microcirculation in the PHT group were better than those in the control group at 30 minutes after reperfusion (RI, 0.54 +/- 0.09 versus 0.65 +/- 0.09; P < .05; time to peak, 31.94 +/- 2.02 versus 48.34 +/- 4.74 seconds; P < .01). Compared to the control group, the aspartate aminotransferase and alanine aminotransferase levels were significantly lower at 30 minutes after reperfusion in the PHT group (P < .05). A pathologic examination revealed a smaller hepatic artery diameter and a depressed vessel wall in the control group. Conclusions The hepatic artery can undergo a transient spasm during the hepatic IRI process, which can exacerbate liver damage. Phentolamine treatment can alleviate hepatic artery spasms, improve liver perfusion, and reduce liver injury by ameliorating the hepatic microcirculation.