C-REACTIVE PROTEIN IN PATIENTS WITH LYMPHATIC FILARIASIS - INCREASED EXPRESSION ON LYMPHOCYTES IN CHRONIC LYMPHATIC OBSTRUCTION
C-REACTIVE PROTEIN IN PATIENTS WITH LYMPHATIC FILARIASIS - INCREASED EXPRESSION ON LYMPHOCYTES IN CHRONIC LYMPHATIC OBSTRUCTION
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DOI：
10.1007/bf00918794
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发表时间：
1991-01-01
影响因子：
9.1
通讯作者：
GAD, AA
中科院分区：
文献类型：
--
作者：
LAL, RB;DHAWAN, RR;GAD, AA
采用酶免疫分析法检测班氏丝虫感染者血清C反应蛋白（CRP）水平。 与无症状微丝蚴血症患者（MF; n = 13）和正常志愿者（NV; n = 29）相比，慢性淋巴病理患者（CP; n = 18）的CRP水平显着升高（P < 0.001）。 班氏丝虫病患者血清CRP水平与循环中含有磷酸胆碱（PC）的丝虫抗原呈负相关（r（s）= -0.37; P < 0.05）。 CP患者CRP结合淋巴细胞百分比显著升高（平均值= 44%; P < 0.001），而MF患者（平均值= 18%）或NV患者（平均值= 3%）。 表面CRP百分比的增加不是由于主要淋巴细胞亚群（CD 5、CD 4、CD 8或CD 19）的异常变化。 CRP与血清CRP无明显相关性，但与携带PC的循环丝虫Ag呈负相关（r（s）= 0.64，P < 0.001）。 抗CRP抗体的生物合成标记和免疫沉淀表明CP患者与MF和CP相比CRP合成的定量差异。 CRP与含有PC的马来丝虫抗原（CRP-BmA）的复合物导致与正常淋巴细胞的结合增加（< 8%），但不接近CP患者中观察到的程度（44%），表明这些患者中CRP的从头合成。 因此，CRP结合淋巴细胞可能代表慢性淋巴阻塞中免疫定向细胞的标志物，并可能在这种疾病的发病机制中发挥作用。
Levels of C-reactive protein (CRP) were evaluated by enzyme immunoassay in patients infected with the filarial parasite Wuchereria bancrofti. Significantly elevated levels of CRP (P < 0.001) were demonstrated in patients with chronic lymphatic pathology (CP; n = 18) compared to patients with asymptomatic microfilaremia (MF; n = 13) and normal volunteers (NV; n = 29). Serum levels of CRP showed an inverse correlation (r(s) = -0.37; P < 0.05) with phosphocholine (PC)-containing filarial antigen that was present in the circulation of patients with bancroftian filariasis. Marked elevations in the percentage of CRP-binding lymphocytes were observed in patients with CP (mean = 44%; P < 0.001) compared to those with MF (mean = 18%) or NV (mean = 3%). The increased percentage of surface CRP was not due to an abnormal change in major lymphocyte subset (CD5, CD4, CD8, or CD19). No significant correlation was noted between surface CRP and serum CRP; however, an inverse correlation was observed between surface CRP and PC-bearing circulating filarial Ag (r(s) = 0.64; P < 0.001). Biosynthetic labeling and immunoprecipitation with anti-CRP antibodies indicated quantitative differences in the synthesis of CRP in patients with CP compared to MF and CP. Complexing of CRP with PC-containing Brugia malayi antigen (CRP-BmA) caused increased binding to normal lymphocytes (< 8%), but not close to the extent seen in patients with CP (44%), suggesting de novo synthesis of CRP in these patients. Thus, the CRP-binding lymphocytes may represent a marker of immunologically committed cells in chronic lymphatic obstruction and may play a role in the pathogenesis of this disease.