Polysaccharide-anchored fatty acid liposome.
Polysaccharide-anchored fatty acid liposome.
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DOI：
10.1016/j.ijpharm.2012.11.013
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发表时间：
2013-01
影响因子：
5.8
通讯作者：
H. W. Tan;M. Misran
中科院分区：
文献类型：
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作者：
H. W. Tan;M. Misran
本文研究了N-pamitoyl壳聚糖（ChP）锚定油酸（OA）脂质体的制备。本研究选择了两种不同类型的具有不同程度酰化（DA）的水溶性ChPs。通过显微镜观察和理化性质分析，证实了OA脂质体表面存在ChPs。原子力显微镜下观察到chp锚定OA （OAChP）脂质体表面的“剥落”效应，证实了脂质体表面存在ChPs层。OAChPs脂质体溶液的表面张力高于OA脂质体溶液。这一结果表明ChPs从空气-水界面去除OA单体。随着ChPs的存在，OA单体每头基最小面积（Amin）的增加进一步证明了OA单体与ChPs疏水部分之间的相互作用。锚定在OA单层上的ChPs增加了OAChP脂质体单层的曲率并减小了脂质体的大小。与未修饰的OA脂质体相比，OAChP脂质体的尺寸减小了30nm。结果表明，锚定的ChPs可以改善OA脂质体的完整性和刚性。
In this study, the preparation of N-pamitoyl chitosan (ChP) anchored oleic acid (OA) liposome was demonstrated. Two different types of water-soluble ChPs with different degrees of acylation (DA) were selected for this study. The presence of ChPs on the surface of OA liposome was confirmed with their micrographs and physicochemical properties. The “peeling off” effect on the surface of the ChP-anchored OA (OAChP) liposomes was observed on the atomic force microscope micrographs and confirmed the presence of the ChPs layer on the liposome surface. The surface tension of the OAChPs liposome solution was found to be higher than that of the OA liposome solution. This result indicated the removal of OA monomer by ChPs from the air–water interface. The increase in the minimum area per headgroup (Amin) of the OA with the presence of ChPs has further proved the interaction between OA monomer and the hydrophobic moieties of the ChPs. The ChPs anchored onto the OA monolayer increased the curvature of the OAChP liposomes monolayer and reduced the liposome size. The size of the OAChP liposomes was reduced by 30nm as compared with the unmodified OA liposome. Results revealed that the anchored ChPs can improve the integrity and rigidity of the OA liposome.