Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3′,5′-cyclic nucleotide phosphodiesterase
Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3′,5′-cyclic nucleotide phosphodiesterase
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DOI：
10.1016/s0378-1119(98)00303-5
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发表时间：
1998-08-17
期刊：
影响因子：
3.5
通讯作者：
Ferguson, K
中科院分区：
文献类型：
--
作者：
Loughney, K;Hill, TR;Ferguson, K
分离出人cgmp结合、cgmp特异性的3′，5′环核苷酸磷酸二酯酶（PDE5A） cdna。由重叠的cdna组装而成的3.1 kb复合DNA序列编码了一个875个氨基酸的蛋白，预计分子量为10012 Da （PDE5A1）。酵母提取物表达人PDE5A1水解cGMP。PDE5A mRNA在主动脉平滑肌细胞、心脏、胎盘、骨骼肌和胰腺中表达，在脑、肝和肺中表达的程度要小得多。一个5'剪接变体PDE5A2编码一个833个氨基酸的蛋白质，在氨基端有8个独特的氨基酸。PDE5A定位于染色体4q 25-27。(C) 1998 Elsevier Science B.V.版权所有
Human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase (PDE5A) cDNAs were isolated. A 3.1-kb composite DNA sequence assembled from overlapping cDNAs encodes an 875-amino-acid protein with a predicted molecular mass of 100 012 Da (PDE5A1). Extracts prepared from yeast expressing human PDE5A1 hydrolyzed cGMP. This activity was inhibited by the selective PDES inhibitors zaprinast and DMPPO, PDE5A mRNA is expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. A 5'-splice variant, PDE5A2, encodes an 833-amino-acid protein with eight unique amino acids at the amino terminus. PDE5A maps to chromosome 4q 25-27. (C) 1998 Elsevier Science B.V. All rights reserved.