Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa
Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa
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DOI：
10.1128/jb.179.10.3127-3132.1997
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发表时间：
1997-05-01
影响因子：
3.2
通讯作者：
Iglewski, BH
中科院分区：
文献类型：
--
作者：
Pesci, EC;Pearson, JP;Iglewski, BH
铜绿假单胞菌通过两个群体感应系统las和rhl根据细胞密度控制几种毒力因子的产生。las系统由转录激活蛋白LasR和LasI组成，LasI指导自诱导剂PAI-1的合成。同样，rhl系统由转录激活蛋白rhr和RhlI组成，RhlI指导自诱导剂PAI-2（以前称为因子2）的合成。为了研究这两个铜绿假单胞菌群体感应系统之间的相互关系，我们将lacZ报告基因融合到lasR、RhlR和rhlA中，并监测这三个基因在不同条件下的表达。每个基因的激活发生在指数期增长,下半年我们还表明,拉斯维加斯群体感应系统控制生殖卫生图书馆群体感应系统在两个方面,第一,我们发现LasR PAI-1 rhlR转录激活,第二,我们表明,PAI-I阻止PAI-2 rhlR绑定,从而抑制rhlA的表达,我们的数据表明,[在rhlR系统施加两个级别的控制,转录和转译后的。
The production of several virulence factors by Pseudomonas aeruginosa is controlled according to cell density through two quorum-sensing systems, las and rhl. The las system is comprised of the transcriptional activator protein LasR and of LasI, which directs the synthesis of the autoinducer PAI-1. Similarly, the rhl system consists of the transcriptional activator protein RhlR and of RhlI, which directs synthesis of the autoinducer PAI-2 (formerly referred to as factor 2), To study the interrelation between the two P. aeruginosa quorum-sensing systems, we fused a lacZ reporter gene to lasR, rhlR, and rhlA and monitored expression of these three genes under various conditions, Our data indicate that lasR and rhlR are expressed in a growth-dependent manner, with activation of each gene occurring during the last half of log-phase growth, We also show that the las quorum-sensing system controls the rhl quorum-sensing system in two ways, First, we found that LasR and PAI-1 activated rhlR transcription, Second, we showed that PAI-I blocked PAI-2 from binding to RhlR, thereby inhibiting the expression of rhlA, Our data thus indicate that the [as system exerts two levels of control on RhlR, transcriptional and posttranslational.