Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group
Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group
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DOI：
10.1016/j.aca.2006.04.059
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发表时间：
2006-09-18
影响因子：
6.2
通讯作者：
Maeda, Mizuo
中科院分区：
文献类型：
--
作者：
Nakanishi, Jun;Kikuchi, Yukiko;Maeda, Mizuo
细胞黏附的控制是基于细胞的药物筛选和细胞过程分析的关键技术。我们开发了一种利用光化学反应在时空上控制细胞黏附的方法。我们用烷基硅氧烷自组装单分子膜修饰玻璃盖片表面，制备了一种细胞培养基质，其中烷基硅氧烷具有光解的2-硝基苄基。牛血清白蛋白(BSA)被吸附到基板上，使其表面对细胞黏附具有惰性。在紫外光照射下，烷基硅氧烷发生光解反应，导致BSA从表面释放。纤维连接蛋白是一种促进细胞黏附的蛋白质，用来覆盖照射区域并使其与细胞黏附。在这种基质上种植细胞会导致它们选择性地附着在受照区域。通过控制照明区的大小，我们形成了比单个细胞更小的细胞粘附点，并定位了细胞的灶性粘连。此外，通过事先照亮底物上形成图案的细胞旁边的区域，我们释放了它们的几何限制，并诱导了迁移和增殖。这些操作是在传统的荧光显微镜下进行的，没有任何额外的仪器。本发明的细胞操作方法将对细胞生物学研究以及细胞阵列的形成有用。(C)2006爱思唯尔B.V.保留所有权利。
Control of cell adhesion is a key technology for cell-based drug screening and for analyses of cellular processes. We developed a method to spatiotemporally control cell adhesion using a photochemical reaction. We prepared a cell-culturing substrate by modifying the surface of a glass coverslip, with a self-assembled monolayer of an alkylsiloxane having a photocleavable 2-nitrobenzyl group. Bovine serum albumin (BSA) was adsorbed onto the substrate to make the surface inert to cell adhesion. When exposed to UV light, the alkylsiloxane underwent a photocleavage reaction, leading to the release of BSA from the surface. Fibronectin, a protein promoting cell adhesion, was added to cover the irradiated regions and made them cell-adhesive. Seeding of cells on this substrate resulted in their selective adhesion to the illuminated regions. By controlling the sizes of the illuminated regions, we formed cell-adhesive spots smaller than single cells and located focal adhesions of the cells. Moreover, by subsequently illuminating the region alongside the cells patterned on the substrate in advance, we released their geometrical confinements and induced migration and proliferation. These manipulations were conducted under a conventional fluorescence microscope without any additional instruments. The present method of cell manipulation will be useful for cell biological studies as well as for the formation of cell arrays. (c) 2006 Elsevier B.V. All rights reserved.