Visualizing a set of olfactory sensory neurons responding to a bile salt
Visualizing a set of olfactory sensory neurons responding to a bile salt
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DOI：
10.1242/jeb.046607
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Hamdani, El Hassan
中科院分区：
文献类型：
--
作者：
Doving, Kjell B.;Hansson, Kenth-Arne;Hamdani, El Hassan
在本研究中，我们将鲫鱼（Carassius Carassius）和褐鳟鱼（Salmo trutta）的嗅觉上皮暴露于右旋糖酐和Alexa染料以及气味剂中。在预先暴露于nocodazole后，染料摄取严重减少，nocodazole是一种微管聚合抑制剂，可损害内吞作用，这支持了气味激活嗅觉受体分子进行内吞作用的假设。胆汁酸牛磺酸石胆酸盐是一种对鱼类有效且特殊的气味剂，其应用导致了稀疏的（不到3%）细胞群的标记，这些细胞群具有典型的纤毛感觉神经元（csn）形态——长树突和细胞体深埋在感觉上皮中。染色遍布感觉神经元，也显示轴突和靶肾小球。染色的轴突在嗅球入口处重新分布，并终止于两个小的目标区域，一个背侧和一个内侧。这些结果与牛头石胆酸盐被少数纤毛感觉神经元特异性检测的观点一致。褐鳟嗅上皮在胆汁酸染色细胞中的应用。对鲫鱼嗅觉器官应用报警激动剂次黄嘌呤-3- n -氧化物引起另一组感觉神经元染色。此外，我们的研究结果表明，气味诱导的染料摄取可以用于识别对特定气味做出反应的嗅觉感觉神经元的亚型，并确定嗅球中这些神经元的目标区域，作为阐明对特定气味做出反应的神经元网络的第一步。
In the present study, we exposed the olfactory epithelia of crucian carp, Carassius carassius, and brown trout, Salmo trutta, to dextran coupled with Alexa dyes together with odorants. Dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. Application of the bile acid taurolithocholate, a potent and specific odorant for fish, resulted in the labelling of a sparse (less than 3%) cell population with the typical morphology of ciliated sensory neurons (CSNs) - long dendrites and cell somata deep in the sensory epithelium. The dye was distributed throughout the sensory neuron, also revealing axons and target glomeruli. Stained axons redistribute at the entrance of the olfactory bulb and terminate in two small target areas, a dorsal and a medial one. These results are consistent with the notion that taurolithocholate is detected specifically by a few ciliated sensory neurons. Application of the olfactory epithelium of brown trout to bile acid stained cells with the appearance of CSNs. Application of an alarm agonist, hypxanthine-3-N-oxide, to crucian carp olfactory organ caused staining of another set of sensory neurons. Furthermore, our results show that odour-induced uptake of a dye can serve to identify the subtype of olfactory sensory neurons responding to a particular odorant, and to pinpoint the target regions of these neurons in the olfactory bulb as a first step to elucidating the neuronal network responding to a particular odour.