Visualization of local afferent inputs to magnocellular oxytocin neurons in vitro
Visualization of local afferent inputs to magnocellular oxytocin neurons in vitro
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体外大细胞催产素神经元局部传入输入的可视化
DOI：
10.1046/j.1460-9568.1999.00620.x
复制
发表时间：
1999
影响因子：
3.4
通讯作者：
D. Theodosis
中科院分区：
文献类型：
--
作者：
P. Jourdain;B. Dupouy;R. Bonhomme;D. Poulain;J. Israel;D. Theodosis
我们最近表明，从出生后大鼠下丘脑获得的器官切片培养物中的催产素（OT）神经元显示出复杂的电活动模式，类似于体内成年大细胞OT神经元的电活动模式。在这里，我们使用此类文化来研究负责此活动的传入输入的身份，特别是起源。光学和共聚焦显微镜的多重免疫染色表明，催产素能神经元的体细胞和树突与许多突触接触，通过它们对突触标记物、突触素或突触蛋白的反应来可视化。许多是 GABA 能的，对谷氨酸脱羧酶或 γ-氨基丁酸 (GABA) 显示出免疫反应性；其他的则富含谷氨酸免疫反应性。这种传入可能分别来自 GABA 或谷氨酸免疫反应性神经元，具有独特的特征形态和拓扑结构。一些多巴胺能受体（酪氨酸羟化酶或多巴胺免疫阳性）影响 OT 神经元；它们源自位于第三脑室的多巴胺阳性神经元。没有检测到去甲肾上腺素能谱。尽管存在胆碱乙酰转移酶（ChAT）免疫反应性神经元，但没有胆碱能接触。最后，我们发现了催产素能突触，通过 OT 相关神经素和突触蛋白的免疫反应鉴定，接触 OT 体细胞和树突。
We recently showed that oxytocin (OT) neurons in organotypic slice cultures obtained from postnatal rat hypothalamus display complex patterns of electrical activity, similar to those of adult magnocellular OT neurons in vivo. Here we used such cultures to investigate the identity and, in particular, the origin of afferent inputs responsible for this activity. Multiple immunostaining with light and confocal microscopy showed that the somata and dendrites of oxytocinergic neurons were contacted by numerous synapses, visualized by their reaction to the synaptic markers, synaptophysin or synapsin. Many were GABAergic, displaying immunoreactivities for glutamic acid decarboxylase or γ‐aminobutyric acid (GABA); others were enriched in glutamate immunoreactivity. Such afferents presumably arose from GABA‐ or glutamate‐immunoreactive neurons, respectively, with distinct and characteristic morphologies and topographies. A few dopaminergic boutons (tyrosine hydroxylase‐ or dopamine‐immunopositive) impinged on OT neurons; they arose from dopamine‐positive neurons located along the third ventricle. No noradrenergic profiles were detected. Despite the presence of choline acetyl‐transferase (ChAT)‐immunoreactive neurons, there were no cholinergic contacts. Lastly, we found oxytocinergic synapses, identified by immunoreaction for OT‐related neurophysin and synapsin, contacting OT somata and dendrites.
谷氨酸和天冬氨酸抗血清的表征。
DOI：
10.1177/36.1.2891743
发表时间：
1988
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Hepler,JR;Toomim,CS;McCarthy,KD;Conti,F;Battaglia,G;Rustioni,A;Petrusz,P
通讯作者：
Petrusz,P