2-Hydrazinoquinoline: A Reactive Matrix for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry to Detect Gaseous Carbonyl Compounds
2-Hydrazinoquinoline: A Reactive Matrix for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry to Detect Gaseous Carbonyl Compounds
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DOI：
10.1255/ejms.1413
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发表时间：
2016-04
影响因子：
1.3
通讯作者：
Y. Shigeri;Takuya Kamimura;M. Ando;K. Uegaki;Hiroaki Sato;F. Tani;R. Arakawa;T. Kinumi
中科院分区：
文献类型：
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作者：
Y. Shigeri;Takuya Kamimura;M. Ando;K. Uegaki;Hiroaki Sato;F. Tani;R. Arakawa;T. Kinumi
考察了2-肼基喹啉作为基质辅助激光解吸电离质谱（MALDI-MS）反应基质的灵敏度、应用范围和反应机理。使用配备有搅拌风扇和用于将MALDI-MS板和挥发性样品移入和移出的窗口的反应室（125 L），2-肼基喹啉对气态醛（甲醛、乙醛、丙醛和正丁醛）和酮（丙酮、甲基乙基酮和甲基异丁基酮）的灵敏度被确定为至少百万分之一（ppm）水平。另一方面，羧酸（甲酸、乙酸、丙酸和丁酸）和酯（乙酸乙酯、乙酸戊酯、乙酸异戊酯和水杨酸甲酯）在MALDI-MS中不能通过2-肼基喹啉检测。除了在气相色谱和液相色谱中用于定量分析羰基化合物的常用衍生试剂2，4-二硝基苯肼之外，2-肼基喹啉在酸性溶液如三氟乙酸中的溶解对于其作为MALDI-MS的反应基质的功能是必需的。
The sensitivity, range of applications, and reaction mechanism of 2-hydrazinoquinoline as a reactive matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) were examined. Using a reaction chamber (125 L) equipped with a stirring fan and a window for moving the MALDI-MS plate and volatile samples in and out, the sensitivities of 2-hydrazinoquinoline to gaseous aldehydes (formaldehyde, acetaldehyde, propionaldehyde, and n-butyraldehyde) and ketones (acetone, methyl ethyl ketone, and methyl isobutyl ketone) were determined to be at least parts per million (ppm) levels. On the other hand, carboxylic acids (formic acid, acetic acid, propionic acid, and butyric acid) and esters (ethyl acetate, pentyl acetate, isoamyl acetate, and methyl salicylate) could not be detected by 2-hydrazinoquinoline in MALDI-MS. In addition to 2,4-dinitrophenylhydrazine, a common derivatization reagent for analyzing carbonyl compounds quantitatively in gas chromatography and liquid chromatography, the dissolution of 2-hydrazinoquinoline in an acidic solution, such as trifluoroacetic acid, was essential for its function as a reactive matrix for MALDI-MS.