Increases in Shoot Tissue Pigments, Glucosinolates, and Mineral Elements in Sprouting Broccoli after Exposure to Short-duration Blue Light from Light Emitting Diodes
Increases in Shoot Tissue Pigments, Glucosinolates, and Mineral Elements in Sprouting Broccoli after Exposure to Short-duration Blue Light from Light Emitting Diodes
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DOI：
10.21273/jashs.138.1.31
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发表时间：
2013-01-01
影响因子：
1.9
通讯作者：
Sams, Carl E.
中科院分区：
文献类型：
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作者：
Kopsell, Dean A.;Sams, Carl E.
微绿叶蔬菜是在第一片真叶出现并新鲜食用后，在根部上方收获的特种绿叶作物。花椰菜（Brassica oleacea var.微绿植物可以积累大量的抗癌芥子油苷，也是其他抗氧化植物化学物质的丰富来源。发光二极管（LED）现在提供了测量窄带波长的光对幼苗生理的影响的能力。类胡萝卜素玉米黄质在植物生理学中被认为是蓝光受体。本研究的目的是测量短持续时间的蓝光对植物化学化合物的影响，这些化合物赋予发芽的西兰花微绿的营养品质。使用生长垫在受控环境中在LED下生长西兰花微绿。将种子培养在浸入去离子水中的垫上，并使用红色（627 nm）/蓝色（470 nm）LED（350 μ mol.m（-2）.s（-1））在23摄氏度的空气温度下在24小时光周期下生长。在第一片真叶出现时，用含42 mg.L-1氮（N）的完全营养液覆盖生长垫。在播种后13天，在收获前，使西兰花小植株在以下任一种下生长：1）红色和蓝色LED光（350 μ mol.m（-2）.s（-1））;或2）蓝色LED光（41 μ mol.m（-2）.s（-1））处理5天。实验重复三次。将冷冻的芽组织冷冻干燥并测量类胡萝卜素、叶绿素、硫代葡萄糖苷和矿物元素。比较两种LED光处理，发现收获前短时间蓝光LED处理显著增加了茎组织中β-胡萝卜素的含量（P
Microgreens are specialty leafy crops harvested just above the roots after the first true leaves have emerged and are consumed fresh. Broccoli (Brassica oleacea var. italica) microgreens can accumulate significant concentrations of cancer-fighting glucosinolates as well as being a rich source of other antioxidant phytochemicals. Light-emitting diodes (LEDs) now provide the ability to measure impacts of narrow-band wavelengths of light on seedling physiology. The carotenoid zeaxanthin has been hypothesized to be a blue light receptor in plant physiology. The objective of this study was to measure the impact of short-duration blue light on phytochemical compounds, which impart the nutritional quality of sprouting broccoli microgreens. Broccoli microgreens were grown in a controlled environment under LEDs using growing pads. Seeds were cultured on the pads submerged in deionized water and grown under a 24-hour photoperiod using red (627 nm)/blue (470 nm) LEDs (350 mu mol.m(-2).s(-1)) at an air temperature of 23 degrees C. On emergence of the first true leaf, a complete nutrient solution with 42 mg.L-1 of nitrogen (N) was used to submerge the growing pads. At 13 days after sowing, broccoli plantlets were grown under either: 1) red and blue LED light (350 mu mol.m(-2).s(-1)); or 2) blue LED light (41 mu mol.m(-2).s(-1)) treatments for 5 days before harvest. The experiment was repeated three times. Frozen shoot tissues were freeze-dried and measured for carotenoids, chlorophylls, glucosinolates, and mineral elements. Comparing the two LED light treatments revealed the short-duration blue LED treatment before harvest significantly increased shoot tissue beta-carotene (P