Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes.
Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes.
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DOI：
10.3390/v12101105
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发表时间：
2020-09-29
期刊：
影响因子：
--
通讯作者：
Chen CH
中科院分区：
文献类型：
--
作者：
Cheng L;Liu WL;Li HH;Su MP;Wu SC;Chen HW;Pan CY;Tsai JJ;Chen CH
登革病毒（DENV）是引起登革热的病原体，主要由埃及伊蚊传播。对受感染蚊子的监测是降低病媒传播疾病爆发风险的综合蚊子控制方法的重要组成部分。然而，目前还没有一种专门的快速检测蚊子中DENV的方法。利用免疫印迹，我们发现从DENV感染的蚊子细胞系和蚊子体分泌的NS 1低于检测阈值。然而，当使用Triton X-100裂解受感染的蚊子时，细胞内的NS 1被释放，然后可以通过NS 1快速测试有效地检测。登革病毒NS 1在经口感染和经胸感染蚊虫体内的分布不同。接下来，我们通过纵向平分蚊子进行敏感性测试;每只蚊子的一半进行NS 1快速测试，而另一半用于qPCR确认。从感染后5天开始，这种改良的检测方法的灵敏度接近90%，而DENV已经从中肠屏障中逃逸。这种适应性测试为蚊子监测提供了一种有价值的，易于使用的工具，这是DENV疾病控制的重要组成部分。
Dengue virus (DENV), the pathogen that causes dengue fever, is mainly transmitted by Aedes aegypti. Surveillance of infected mosquitoes is a major component of integrated mosquito control methods for reducing the risk of vector-born disease outbreaks. However, a specialized rapid test for DENV detection in mosquitoes is not currently available. Utilizing immunoblotting, we found that the secretion of NS1 from both a DENV-infected mosquito cell line and mosquito bodies was below the detection threshold. However, when Triton X-100 was used to lyse infected mosquitoes, intracellular NS1 was released, and could then be effectively detected by the NS1 rapid test. The distribution of DENV NS1 in intrathoracically infected mosquitoes was different from that of orally infected mosquitoes. Next, we performed sensitivity tests by bisecting mosquitoes longitudinally; one half of each mosquito was subjected to the NS1 rapid test while the other half was used for qPCR confirmation. This modified test had a sensitivity of nearly 90% from five days post-infection onwards, while DENV had escaped from the midgut barrier. This adapted test offers a valuable, easy-to-use tool for mosquito surveillance, which is a crucial component of DENV disease control.
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DOI：
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发表时间：
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影响因子：
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