A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box-Behnken experimental design and kinetics
A green ultrasound-assisted extraction process for the recovery of antioxidant polyphenols and pigments from onion solid wastes using Box-Behnken experimental design and kinetics
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DOI：
10.1016/j.indcrop.2015.09.039
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发表时间：
2015-12-23
影响因子：
5.9
通讯作者：
Makris, Dimitris P.
中科院分区：
文献类型：
--
作者：
Katsampa, Photene;Valsamedou, Evdokea;Makris, Dimitris P.
以洋葱固体废弃物为原料，采用超声波辅助固液萃取法提取富含多酚和色素的抗氧化提取物。所用浸提介质不含常规挥发性有机溶剂，由水和甘油组成。首先采用Box-Behnken实验设计优化甘油浓度（C-gl）和液固比（R-L/s），然后通过动力学进一步评估提取效率。结果表明，最佳工艺条件为C-gl = 90%（w/v），R-L/s = 90 mLg（-1），在此条件下，总多酚和总色素的提取符合一级动力学规律。总多酚和总色素的最大有效扩散（D-e）值分别为9.28 x 10（-11）和13.22 x 10（-11）m（2）s（-1），相应的活化能（E-a）分别为31.52和12.11 kJ mol（-1）。还原能力（P-R）随着提取温度的升高而增加，并且在80 ℃下获得的提取物的P-R值为490 μ mol抗坏血酸当量/g干重。液相色谱-二极管阵列-质谱分析表明，初步鉴定的主要多酚类物质为槲皮素4 '-O-葡萄糖苷和槲皮素，并伴有其他槲皮素苷、氰化物3-O-葡萄糖苷和一系列槲皮素降解氧化产物。(C)2015 Elsevier B. V.版权所有。
Onion solid wastes (OSW) were used as raw material to produce polyphenol- and pigment-enriched extracts with antioxidant activity, using ultrasound-assisted solid-liquid extraction. The extraction medium used was free from conventional volatile organic solvents, and it was composed of water and glycerol. Glycerol concentration (C-gl) and liquid-to-solid ratio (R-L/s) were first optimised employing Box-Behnken experimental design and then extraction efficiency was further assessed through kinetics. The optimal conditions were found to be C-gl = 90% (w/v) and R-L/s = 90 mLg(-1), and under these conditions the extraction of total polyphenols and total pigments was shown to obey first-order kinetics. Maximal effective diffusion (D-e) values were 9.28 x 10(-11) and 13.22 x 10(-11) m(2) s(-1), for total polyphenols and total pigments, respectively, while the corresponding activation energies (E-a) were 31.52 and 12.11 kJ mol(-1). The reducing power (P-R) increased as a response to increasing extraction temperature and the extract obtained at 80 degrees C had P-R value of 490 mu mol ascorbic acid equivalents per g of dry weight. Liquid chromatography-diode array-mass spectrometry analysis of this extract showed that the major polyphenols tentatively identified were quercetin 4'-O-glucoside and quercetin, accompanied by other quercetin glycosides, cyaniding 3-O-glucoside and a series of quercetin degradation and oxidation products. (C) 2015 Elsevier B.V. All rights reserved.