AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis.
AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis.
复制标题
AMID 介导腺苷诱导的不依赖 caspase 的 HuH-7 细胞凋亡。
DOI：
10.1159/000325203
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Nishizaki,Tomoyuki
中科院分区：
文献类型：
--
作者：
Yang,Dongqin;Yaguchi,Takahiro;Nagata,Tetsu;Gotoh,Akinobu;Dovat,Sinisa;Song,Chunhua;Nishizaki,Tomoyuki
Background/Aims: The mechanism underlying extracellular adenosine-induced caspase-independent apoptosis in HuH-7 human hepatoma cells is not fully understood. The present study investigated the role for apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death (AMID) in the pathway. Methods: To see the implication of AMID in adenosine-induced HuH-7 cell apoptosis, real-time reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescent cytochemistry, time-laps GFP monitoring, cell cycle analysis, flow cytometry, Western blotting, cell viability assay, and TUNEL staining were carried out. Results: Adenosine upregulated AMID expression in HuH-7 cells, and translocated AMID from the cytosol into the nucleus. Adenosine induced HuH-7 cell apoptosis, and the effect was further enhanced by overexpressing AMID. Adenosine-induced HuH-7 cell apoptosis, alternatively, was inhibited by knocking-down AMID. Conclusion: The results of the present study provide evidence for AMID as a critical factor for adenosine-induced caspase-independent HuH-7 cell apoptosis.
