Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis
Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis
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DOI：
10.1126/scisignal.2001140
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发表时间：
2010-09-28
影响因子：
7.3
通讯作者：
Smith, Steven M.
中科院分区：
文献类型：
--
作者：
Che, Ping;Bussell, John D.;Smith, Steven M.
适应胁迫的能力使植物能够在不利的环境条件下生长和发育。由内质网（ER）应激触发的调节性膜内蛋白水解（RIP）介导了某些形式的应激信号传导。类甾醇（BR）参与植物对逆境的适应，但尚未发现其激活机制。在这里，我们揭示了ER应激信号和BR介导的生长和应激适应之间的联系。拟南芥转录因子bZIP 17和bZIP 28从内质网通过高尔基体易位，在高尔基体中它们被位点2蛋白酶蛋白水解切割并释放以易位到细胞核中。应力，包括热和蛋白质糖基化的抑制，增加这两个bZIPs的易位到细胞核。这些核定位的bZIPs不仅激活ER伴侣基因，而且激活BR信号传导，这是胁迫适应和生长所必需的。因此，这些bZIPs链接ER胁迫和BR信号传导，这可能是植物生长和胁迫响应可以整合的机制。
The ability to acclimate to stresses enables plants to grow and develop under adverse environmental conditions. Regulated intramembrane proteolysis (RIP) triggered by endoplasmic reticulum (ER) stress mediates some forms of stress signaling. Brassinosteroids (BRs) have been implicated in plant adaptation to stress, but no mechanisms for activation have been discovered. Here, we reveal a connection between ER stress signaling and BR-mediated growth and stress acclimation. Arabidopsis transcription factors bZIP17 and bZIP28 were translocated from the ER through the Golgi, where they were proteolytically cleaved by site 2 protease and released to translocate into the nucleus. Stresses, including heat and inhibition of protein glycosylation, increased translocation of these two bZIPs to the nucleus. These nuclear-localized bZIPs not only activated ER chaperone genes but also activated BR signaling, which was required for stress acclimation and growth. Thus, these bZIPs link ER stress and BR signaling, which may be a mechanism by which plant growth and stress responses can be integrated.