Discovery of a phoretic association between Bursaphelenchus doui (Nematoda: Aphelenchoididae) and Monochamus saltuarius and Acalolepta sejuncta (Coleoptera: Cerambycidae)
Discovery of a phoretic association between Bursaphelenchus doui (Nematoda: Aphelenchoididae) and Monochamus saltuarius and Acalolepta sejuncta (Coleoptera: Cerambycidae)
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DOI：
10.1163/15685411-00003334
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发表时间：
2020-07-01
期刊：
影响因子：
1.2
通讯作者：
Kanzaki, Natsumi
中科院分区：
文献类型：
--
作者：
Aikawa, Takuya;Ozawa, Sota;Kanzaki, Natsumi
研究日本东北部松材线虫属线虫与天牛甲虫之间的泳动关系。山田镇和平内镇的两片松树林中放置了赤松原木作为甲虫产卵地。产卵后 1-2 年，Monochcunus saltuarius 成虫从放置在 Yamada 的原木中出现，Acalolepta sejuncta 和 A.fracurix 成虫从放置在 Hiranai 的原木中出现。从其中两个物种（M. saltuarius 和 A. sejuncta）中回收了伞滑刃线虫。对内部转录间隔区 1 和 2 以及 5.8S 核糖体 RNA 基因的形态计量学和系统发育分析表明，这两个线虫种群都是 B. doui，此前仅知道该线虫使用 M. subfasciatus 和 A.fraguhaatrix 作为载体。因此，这项研究表明，该线虫在田间利用至少属于两个属的四种甲虫作为媒介。
To investigate the phoretic association between nematodes in the genus Bursaphelenchus and cerambycid beetles in northeastern Japan. Pinus densiflora logs were placed in two pine forests in the towns of Yamada and Hiranai as beetle oviposition sites. At 1-2 years after oviposition, adults of Monochcunus saltuarius emerged from the logs placed in Yamada and adults of Acalolepta sejuncta and A. fraudcurix emerged from the logs placed in Hiranai. and Bursaphelenchus nematodes were recovered from two of these species (M. saltuarius and A. sejuncta). Morphometrics and a phylogenetic analysis of the internal transcribed spacer regions 1 and 2 and the 5.8S ribosomal RNA gene indicated that both of these nematode populations were B. doui, which has previously only been known to use M. subfasciatus and A. fraudatrix as vectors. Therefore, this study demonstrated that this nematode utilises at least four species of beetles belonging to two genera as vectors in the field.