Coherent patterns in bacterial growth, growth efficiency, and leucine metabolism along a northeastern Pacific inshore-offshore transect
Coherent patterns in bacterial growth, growth efficiency, and leucine metabolism along a northeastern Pacific inshore-offshore transect
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DOI：
10.4319/lo.2011.56.1.0001
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发表时间：
2011-01-01
影响因子：
4.5
通讯作者：
Gasol, Josep M.
中科院分区：
文献类型：
--
作者：
del Giorgio, Paul A.;Condon, Robert;Gasol, Josep M.
我们研究了细菌生长、生产、呼吸、生长效率（BGE）和细菌亮氨酸呼吸和C-亮氨酸产率（即，转换因子[CF]）。浮游生物呼吸沿着样带平均为1.15 +/- 0.16 μ g C L-1 h（-1），在沿海上升流区达到峰值。过滤组分中的呼吸占总呼吸的79%，主要由细菌生物量组成。我们使用的不同方法收敛到13% +/-1%的平均BGE，在生产力较高的沿海地区达到20%以上的峰值，在贫营养环流沃茨下降到5%以下。细菌C代谢的各个方面之间存在总体一致性：BGE低的社区也倾向于具有低增长率和高亮氨酸-胸苷掺入比。BGE中的模式在单一化合物水平上得到反映，并且在大多数贫营养位点中，细菌倾向于快速呼吸大部分（20-75%）的亮氨酸，所述亮氨酸被吸收并且具有最低的C-亮氨酸产率，这表明批量BGE和生长中的模式也适用于单独的底物。细菌生长是碳消耗和BGE的函数;细菌碳代谢的这两个方面不一定是共变的，它们受到不同的调节。C消耗、生长、BGE和亮氨酸代谢的模式都反映了细菌对能量限制的基本生理反应，能量限制是由于与贫养生活相关的高维持成本。
We investigated the patterns in bacterial growth, production, respiration, growth efficiency (BGE), and bacterial leucine respiration and C-to-leucine yield (i.e., conversion factor [CF]) along a transect off the coast of Oregon. Plankton respiration along the transect averaged 1.15 +/- 0.16 mu g C L-1 h(-1), peaking in the coastal upwelling region. The respiration in the filtered fraction, which was dominated by bacterial biomass, accounted for 79% of the total respiration. The different approaches that we used converged to an average BGE of 13% +/- 1%, with peaks of over 20% in the more productive coastal areas and values declining to below 5% toward the oligotrophic gyre waters. There was overall coherence between the various aspects of bacterial C metabolism: communities with low BGE also tended to have low growth rates and high leucine-to-thymidine incorporation ratios. The patterns in BGE were mirrored at the single compound level, and in the most oligotrophic sites, bacteria tended to quickly respire a large fraction (20-75%) of the leucine that was taken up and had the lowest C-to-leucine yield, suggesting that the patterns in bulk BGE and growth also apply to individual substrates. Bacterial growth was a function of both C consumption and BGE; these two aspects of bacterial C metabolism do not necessarily covary, and they are regulated differently. The patterns in C consumption, growth, BGE, and leucine metabolism all reflect the basic physiological response of bacteria to energy limitation due to high maintenance costs associated with life in oligotrophy.