The use of physical hydrogels of chitosan for skin regeneration following third-degree burns
The use of physical hydrogels of chitosan for skin regeneration following third-degree burns
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DOI：
10.1016/j.biomaterials.2007.04.021
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发表时间：
2007-08-01
期刊：
影响因子：
14
通讯作者：
Domard, Alain
中科院分区：
文献类型：
--
作者：
Boucard, Nadge;Viton, Christophe;Domard, Alain
皮肤修复是组织工程的一个重要领域，特别是在大面积三度烧伤的情况下，目前的治疗方法仍不足以促进令人满意的皮肤再生。仅由壳聚糖和水组成的仿生双层物理水凝胶被加工并应用于全层烧伤的治疗。该研究的目的是评估这种材料是否被宿主生物体完全接受并允许在体内重建有限面积的三度烧伤皮肤。由刚性保护凝胶构成的第一层确保了良好的机械性能和气体交换。第二层柔软且有弹性，使材料能够遵循伤口的几何形状，并确保良好的表面接触。为了进行比较，还考虑了壳聚糖的高粘度溶液。在第9、17、22、100和293天对猪的皮肤和活组织检查进行兽医实验，通过组织学和免疫组织化学进行分析。每次仅使用一种壳聚糖材料。所有结果表明壳聚糖材料具有良好的耐受性并促进良好的组织再生。它们诱导炎症细胞迁移和血管生成活性，有利于新组织的高血管化。第22天，肉芽组织下合成I型和IV型胶原，并观察到真皮-表皮连接处的形成。 100 天后，新组织与原生皮肤非常相似，特别是在美观和灵活性方面。 (c) 2007 Elsevier Ltd. 保留所有权利。
Skin repair is an important field of the tissue engineering, especially in the case of extended third-degree burns, where the current treatments are still insufficient in promoting satisfying skin regeneration. Bio-inspired bi-layered physical hydrogels only constituted of chitosan and water were processed and applied to the treatment of full-thickness burn injuries. The aim of the study was at assessing whether this material was totally accepted by the host organism and allowed in vivo skin reconstruction of limited area third-degree burns. A first layer constituted of a rigid protective gel ensured good mechanical properties and gas exchanges. A second soft and flexible layer allowed the material to follow the geometry of the wound and ensured a good superficial contact. To compare, highly viscous solutions of chitosan were also considered. Veterinary experiments were performed on pig's skins and biopsies at days 9, 17, 22, 100 and 293, were analysed by histology and immuno-histochemistry. Only one chitosan material was used for each time. All the results showed that chitosan materials were well tolerated and promoted a good tissue regeneration. They induced inflammatory cells migration and angiogenetic activity favouring a high vascularisation of the neo-tissue. At day 22, type I and IV collagens were synthesised under the granulation tissue and the formation of the dermal-epidermal junction was observed. After 100 days, the new tissue was quite similar to a native skin, especially by its aesthetic aspect and its great flexibility. (c) 2007 Elsevier Ltd. All rights reserved.