Sequencing for oxidative DNA damage at single-nucleotide resolution with click-code-seq v2.0.
Sequencing for oxidative DNA damage at single-nucleotide resolution with click-code-seq v2.0.
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DOI：
10.1039/d3cc02699j
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发表时间：
2023-07
影响因子：
4.9
通讯作者：
Songjun Xiao;A. Fleming;C. Burrows
中科院分区：
文献类型：
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作者：
Songjun Xiao;A. Fleming;C. Burrows
DNA核苷酸的氧化损伤具有许多细胞结果，这些结果可以通过测序方法的发展来帮助。在本文中，重新开发了先前报道的用于对单个损伤类型进行测序的点击-代码-测序方法，以通过对方案进行简单的改变来实现对许多损伤类型的测序（即，click-code-seq v2.0）。
Oxidative damage to DNA nucleotides has many cellular outcomes that could be aided by the development of sequencing methods. Herein, the previously reported click-code-seq method for sequencing a single damage type is redeveloped to enable the sequencing of many damage types by making simple changes to the protocol (i.e., click-code-seq v2.0).