First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.
First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.
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DOI：
10.1016/j.fsi.2010.08.014
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发表时间：
2010-12
影响因子：
4.7
通讯作者：
M. De Zoysa;C. Nikapitiya;Youngdeuk Lee;Sukkyoung Lee;Chulhong Oh;I. Whang;Sang-Yeop Yeo;C. Choi;Jehee Lee
中科院分区：
文献类型：
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作者：
M. De Zoysa;C. Nikapitiya;Youngdeuk Lee;Sukkyoung Lee;Chulhong Oh;I. Whang;Sang-Yeop Yeo;C. Choi;Jehee Lee
转录激活的调控是基因表达的一个基本和关键点。本研究中，我们首次在软体动物中发现了一个新的盘鲍转录因子激活蛋白-1（Ap-1）基因。通过鲍鱼标准化cDNA文库的同源性筛选鉴定了该基因。克隆的AbAp-1由945 bp的编码区组成，编码含有315个氨基酸的推定蛋白。AbAp-1基因由一个特征性的Jun转录因子结构域和一个高度保守的碱性亮氨酸拉链（bZIP）签名组成，类似于已知的Ap-1基因。AbAp-1与鱼类（红足东方鲀）、人类和昆虫（肩突硬蜱）Ap-1的氨基酸同源性分别为46%、43%和40%。定量真实的时间RT-PCR分析证实，AbAp-1基因的表达是在所有选定的组织组成。AbAp-1在细菌（溶藻弧菌、副溶血弧菌和单核细胞增多性李斯特菌）攻击后的鳃中上调;并且，在病毒性出血性败血症病毒（VHSV）攻击后的血细胞和鳃中上调。壳损伤和组织损伤也增加了外套膜中Ap-1以及其他转录因子（NF-kB、LITAF）和促炎性TNF-α的转录水平。所有的结果考虑，鉴定和基因表达数据表明，鲍鱼Ap-1是一个重要的调节剂，在先天免疫反应对细菌和病毒，以及在炎症反应在组织损伤。此外，在不同的外部刺激下刺激Ap-1可能有助于了解Ap-1生物学及其下游靶基因，特别是在鲍鱼类软体动物中。
The regulation of transcriptional activation is an essential and critical point in gene expression. In this study, we describe a novel transcription factor activator protein-1 (Ap-1) gene from disk abalone Haliotis discus discus (AbAp-1) for the first time in mollusk. It was identified by homology screening of an abalone normalized cDNA library. The cloned AbAp-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. The AbAp-1 gene is composed of a characteristic Jun transcription factor domain and a highly conserved basic leucine zipper (bZIP) signature similar to known Ap-1 genes. The AbAp-1 shares 46, 43 and, 40% amino acid identities with fish (Takifugu rubripes), human and insect (Ixodes scapularis) Ap-1, respectively. Quantitative real time RT-PCR analysis confirmed that AbAp-1 gene expression is constitutive in all selected tissues. AbAp-1 was upregulated in gills after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes) challenge; and, upregulated in hemocytes and gills by viral hemorrhagic septicemia virus (VHSV) challenge. Shell damage and tissue injury also increased the transcriptional level of Ap-1 in mantle together with other transcription factors (NF-kB, LITAF) and pro-inflammatory TNF-α. All results considered, identification and gene expression data demonstrate that abalone Ap-1 is an important regulator in innate immune response against bacteria and virus, as well as in the inflammatory response during tissue injury. In addition, stimulation of Ap-1 under different external stimuli could be useful to understand the Ap-1 biology and its downstream target genes, especially in abalone-like mollusks.