High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes
High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes
复制标题
DOI：
10.1007/s10800-011-0319-7
复制
发表时间：
2011-08-01
影响因子：
2.9
通讯作者：
Nelson, Andrew
中科院分区：
文献类型：
--
作者：
Coldrick, Zachary;Penezic, Abra;Nelson, Andrew
成功研制了一种用于水样中脂类和生物膜活性化合物快速在线筛选的传感系统。该传感器由一个液流电池和片上汞对铂（Pt/Hg）工作电极、铂（Pt）辅助电极和伪参比电极组成。为了优化系统性能，进行了以下实验：(i)分别在Pt/Hg电极上沉积和去除磷脂层；（ii）电极尺寸对信号的影响；（iii）监测流动池中沉积的不同磷脂；（iv）检测磷脂单层与代表性化合物的相互作用。结果表明：(1)成功实现了汞(Hg)/磷脂体系的小型化和坚固化，(2)快速循环伏安法促进了水相DOPC分散在Hg上重复形成二油基磷脂酰胆碱（DOPC）单层，(3)该装置选择性地响应注入电解质流动的有机化合物。
A sensing system for the rapid on-line screening of lipids and biomembrane active compounds in water samples has been successfully developed. The sensor consists of a flow cell and incorporated wafer-based device with on-chip mercury on platinum (Pt/Hg) working electrode and platinum (Pt) auxiliary and pseudo-reference electrodes. To optimise system performance, the following experiments were carried out: (i) Deposition and removal of phospholipid layers on and from Pt/Hg electrodes respectively, (ii) Effect of electrode size on signal, (iii) Monitoring of different phospholipids deposited in flow cell and, (iv) Detection of phospholipid monolayer interaction with representative compounds. The results showed that: (i) Miniaturisation and ruggedisation of the mercury (Hg)/phospholipid system has been successfully achieved, (ii) Rapid cyclic voltammetry facilitates repetitive dioleoyl phosphatidylcholine (DOPC) monolayer formation on Hg from aqueous DOPC dispersion and, (iii) The device responds selectively to organic compounds injected into electrolyte flow.