Unique archaeal assemblages in the Arctic Ocean unveiled by massively parallel tag sequencing
Unique archaeal assemblages in the Arctic Ocean unveiled by massively parallel tag sequencing
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DOI：
10.1038/ismej.2009.23
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发表时间：
2009-07-01
期刊：
影响因子：
11
通讯作者：
Lovejoy, Connie
中科院分区：
文献类型：
--
作者：
Galand, Pierre E.;Casamayor, Emilio O.;Lovejoy, Connie
北冰洋在控制太平洋和大西洋之间的营养平衡方面发挥着关键作用。古生菌是氮循环和营养循环的关键参与者，但在北冰洋对它们的群落组成研究很少。在这里，我们使用16S rRNA基因V6区域的大规模平行标签测序来表征来自北极表层和深层水团的古菌组合。这种方法提供了非常高的自然群落覆盖率，使得能够准确地描述古生物组合。这是迄今为止报道的第一个通过标签测序对古菌群落进行分类描述的方法，这表明有可能将低于门水平的身份分配给大多数(95%)古生菌V6标签，并表明标签测序是解决环境中特定微生物多样性和分布的有力工具。总体而言，海洋I类群是北冰洋中含量最丰富的类群，占所有标签的27%至63%。第三类Eurya chaeota在深水团中更为丰富，是北冰洋中部大西洋深层最大的古生物类群。而沿海表层水域则容纳了更多的第二类Eurya chaeota。此外，主宰表层水域的第II类层序与在深水中检测到的第II类层序不同，这表明在密切相关的类群中存在功能差异。我们的结果首次揭示了由Eurya chaeota组III占优势的古菌群落，并显示了海洋北极古生物的生物地理特征。《ISME期刊》(2009年)3860869；doi：10.1038/ismej.2009.23；3月26日在线发布
The Arctic Ocean plays a critical role in controlling nutrient budgets between the Pacific and Atlantic Ocean. Archaea are key players in the nitrogen cycle and in cycling nutrients, but their community composition has been little studied in the Arctic Ocean. Here, we characterize archaeal assemblages from surface and deep Arctic water masses using massively parallel tag sequencing of the V6 region of the 16S rRNA gene. This approach gave a very high coverage of the natural communities, allowing a precise description of archaeal assemblages. This first taxonomic description of archaeal communities by tag sequencing reported so far shows that it is possible to assign an identity below phylum level to most (95%) of the archaeal V6 tags, and shows that tag sequencing is a powerful tool for resolving the diversity and distribution of specific microbes in the environment. Marine group I Crenarchaeota was overall the most abundant group in the Arctic Ocean and comprised between 27% and 63% of all tags. Group III Euryarchaeota were more abundant in deep-water masses and represented the largest archaeal group in the deep Atlantic layer of the central Arctic Ocean. Coastal surface waters, in turn, harbored more group II Euryarchaeota. Moreover, group II sequences that dominated surface waters were different from the group II sequences detected in deep waters, suggesting functional differences in closely related groups. Our results unveiled for the first time an archaeal community dominated by group III Euryarchaeota and show biogeographical traits for marine Arctic Archaea. The ISME Journal (2009) 3, 860-869; doi: 10.1038/ismej.2009.23; published online 26 March 2009